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| 短葶山麦冬茎尖培养快速繁殖技术研究 | |
| 引用本文： | 万学锋,赖钟雄,陈菁瑛. 短葶山麦冬茎尖培养快速繁殖技术研究[J]. 福建农业学报, 2011, 26(5): 778-782 |
| 作者姓名： | 万学锋 赖钟雄 陈菁瑛 |
| 作者单位： | 1. 福建省农业科学院农业生物资源研究所,福建福州350003;福建省农业科学院药用植物研究中心,福建福州 350003;福建农林大学园艺植物生物工程研究所,福建福州350002 2. 福建农林大学园艺植物生物工程研究所,福建福州,350002 3. 福建省农业科学院农业生物资源研究所,福建福州350003;福建省农业科学院药用植物研究中心,福建福州 350003 |
| 基金项目： | 福建省科技平台建设项目，国家“十一五”科技支撑计划 |
| 摘 要： | 以短葶山麦冬茎尖为外植体,通过对其采取不同消毒时间、茎尖诱导分化、壮苗与生根及移栽条件等快速繁殖条件进行研究.结果表明:0.1％ HgCl2消毒时间为12 min;短葶山麦冬茎尖分化培养基MS+ BA 2.0mg·L-1+ NAA0.2 mg·L-1,茎尖分化率高,最高分化率达到90％.丛生芽增殖培养基选择MS+ BA... |
| 关 键 词： | 短葶山麦冬 茎尖培养 快速繁殖 |
Micropropagation for Liriope muscari (Desne.) Bailey |
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| WAN Xue-feng,LAI Zhong-xiong,CHEN Jing-ying. Micropropagation for Liriope muscari (Desne.) Bailey[J]. Fujian Journal of Agricultural Sciences, 2011, 26(5): 778-782 | |
| Authors: | WAN Xue-feng LAI Zhong-xiong CHEN Jing-ying |
| Affiliation: | 1.Agricultural Bio-resources Institute of Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;2.Medicinal Plant Research Center,Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350003,China;3.Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
| Abstract: | Shoot tips from Liriope muscari(Desne.) Bailey were used as explants to grow on media with varied sterilization time,under induced differentiation,as well as different strengthening and rooting treatments,and transplantation conditions.The shoot tips were cut from the plants and cultured on MS medium containing 2.0 mg·L-1 BA and 0.2 mg·L-1 NAA.The highest differentiating rate of 90% was achieved.The medium with 4.0 mg·L-1BA and 0.2 mg·L-1 NAA was suitable for multiple-shoot proliferation.A certain concentrations of BA promoted the shoot differentiation.The optimized medium for seeding and rooting was when MS was mixed with 0.5 mg·L-1 IBA,which improved the root coefficient to 6.87.Peat soil was found to be the best medium material for the transplantation. |
| Keywords: | Liriope muscari(Desne.) Bailey shoot tip culture miacropropagation |
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