不同来源红木材料的RAPD 分析及分类学探讨

 以2 个生态型木材料为对照, 对在各原产地收集到的14 个红木材料进行55 个随机引物的RAPD 反应, 共产生632 条稳定的扩增带, 其中427 条具有多态性。按扩增带的有无列出二元数据矩阵,利用NTSYS􀀁pc 计算Nei 氏相似系数及遗传距离, 利用SAS 软件进行主成分分析。结果表明: 红木是木的变种, 供试的14 个红木材料分属4 类, 其突变途径可能是一个复杂的过程, 存在着木不同生态型间、木与红木之间, 红木之间的相互作用。

RAPD Analysis and Taxology Discussion on Loropetalum chinense var. rubrum Materials from Different Fountainhead Areas

Contrasted with 2 different Loropetalum chinense ecotypes,fourteen Loropetalum chinense var.rubrum materials representing almost all the types could be were analyzed by RAPD in this paper.Fifty-five random primers produced 632 steady amplified bands in total,among them 427 bands were polymorphic.Strong and clear bands were scored for their presence or absence in a binary data matrix.Nei's similarity coefficients and genetic distances were calculated by NTSYS-pc software,and principal components analysis was done by SAS software.The result demonstrated that L.chinense var.rubrum is a mutant of L.chinense on molecular level and 14 L. chinense var.rubrum materials were classified into 4 groups.The mutant pathway of 4 groups is possibly a complicated process,including interactions between different ecotypes L.chinense,interactions between L. chinense and L.chinense var.rubrum,and ineractions between different L.chinense var.rubrum.