A rapid method for the quantification of steroidal glycoalkaloids by reversed phase HPLC

Summary Methods were developed for clean-up of potato tuber extracts on solid phase extraction CN-cartridges and for analysis of steroidal glycoalkaloids by reversed phase HPLC. The alkaloids α-solanine, α-chaconine, α-solasonine and α-solamargine could be separated on C₈ or C₁₈ reversed phase columns using a mobile phase of acetonitrile/Tris-buffer (3∶2, v/v). The analysis time of tuber extracts containing these alkaloids was less then 4 min, if the pH of the mobile phase was adjusted to 7.4 and run at a flow rate of 0.5 ml/min.