| 甜叶菊种子丛生芽诱导和植株再生 |
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| 引用本文： | 袁学军,陈光宙,李艳丽.甜叶菊种子丛生芽诱导和植株再生[J].草原与草坪,2010,30(6):55-57. |
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| 作者姓名： | 袁学军 陈光宙 李艳丽 |
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| 作者单位： | 海南琼州学院,生科院,海南,三亚,572000 |
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| 摘 要： | 以甜叶菊种子为外植体,成功建立了快繁再生体系。基本培养基为MS,在添加了6-BA1.0mg/L和NAA0.5 mg/L的培养基上最适于丛生芽诱导,诱导率为98.6%;最佳壮苗培养基为MS+6-BA0.5 mg/L+KT0.5 mg/L+GA0.2 mg/L+NAA0.1 mg/L;试管苗最佳生根培养基为MS+NAA0.2 mg/L,生根率达到99.5%;生根的试管苗移栽到装有田园土的土盆中,其存活率达到82.4%。本试验为甜叶菊快速繁殖工作奠定了良好的实验基础。
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| 关 键 词： | 甜叶菊 种子 丛生芽 快繁 |
Development of a plant rapid propagation system from seed-derived cluster buds of sweet Stevia rebaudianum |
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| YUAN Xue-jun,CHEN Guang-zhou,LI Yan-li.Development of a plant rapid propagation system from seed-derived cluster buds of sweet Stevia rebaudianum[J].Grassland and Turf,2010,30(6):55-57. |
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| Authors: | YUAN Xue-jun CHEN Guang-zhou LI Yan-li |
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| Institution: | YUAN Xue-jun,CHEN Guang-zhou,LI Yan-li (College of Life Science,Qiongzhou University,Sanya 572000,China) |
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| Abstract: | Plant rapid propagation systems were successfully established with seed of Stevia rebaudianum as explant.The optimum medium for cluster buds induction was MS medium with 6-BA(1.0 mg/L)and NAA(0.5 mg/L),and the cluster buds induction rate was 98.6%;the optimum medium for shoot growth was MS medium with 6-BA(0.5 mg/L),KT(0.5 mg/L),GA(0.2 mg/L)and NAA(0.1 mg/L);The rooting rate was 99.5% on optimum rooting medium with NAA(0.2 mg/L).The survival rate of transplanting plant was 82.4% after transferred to pots wi... |
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| Keywords: | Stevia rebaudianum seed cluster buds plant rapid propagation |
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