| 绵羊MKRN3基因多态性与产羔数的关联分析 |
| |
| 作者单位： | ;1.中国农业科学院北京畜牧兽医研究所;2.农业部动物遗传育种与繁殖重点实验室;3.扬州大学动物科学与技术学院;4.河南科技大学动物科技学院 |
| |
| 摘 要： | 本实验旨在探究绵羊MKRN3基因g.275981C>T与g.276999C>T位点多态性与绵羊产羔数之间的关系,以期为绵羊高繁殖力分子育种提供新的遗传标记。利用全基因组重测序结合Sequenom MassARRAY~?SNP技术对常年发情绵羊品种(小尾寒羊、策勒黑羊和湖羊)和季节性发情绵羊品种(滩羊、苏尼特羊和草原型藏羊)MKRN3基因2个多态位点多态性进行检测,并与小尾寒羊产羔数进行关联分析。结果表明:MKRN3基因g.275981C>T位点与g.276999C>T位点均存在3种基因型;g.276999C>T位点基因型频率和等位基因频率在2种发情模式绵羊品种间差异均达到极显著水平(P<0.01);g.275981C>T位点在6个绵羊品种中均表现为低度多态(PIC<0.25),g.276999C>T位点在6个绵羊品种中均表现为中度多态(0.25T位点在苏尼特羊和策勒黑羊中均处于哈代温伯格平衡状态(P>0.05),g.276999C>T位点在6个绵羊品种中均处于哈代温伯格平衡状态;关联分析表明,g.275981C>T和g.276999C>T位点不同基因型与小尾寒羊第1、2、3胎产羔数均无显著关联。可见,MKRN3基因2个多态位点均不适合用于小尾寒羊产羔数选育。
|
| 关 键 词： | 绵羊 MKRN3基因 SNP分型 产羔数 |
Polymorphism of Sheep MKRN3 Gene and Its Association with Litter Size |
| |
| Institution: | ,Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences,College of Animal Science and Technology, Yangzhou University,College of Animal Science and Technology, Henan University of Science and Technology |
| |
| Abstract: | To elucidate the association between MKRN3 gene polymorphism and litter size in sheep. Year-round estrous sheep breeds(Small Tail Han, Cele Black and Hu sheep) and seasonal estrous sheep breeds(Tan, Sunite and Prairie Tibetan sheep) were selected and Sequenom MassARRAY~?SNP assay was applied to genotype g.275981 C>T and g.276999 C>T loci of MKRN3 gene. Then the association was analyzed between MKRN3 and litter size in Small Tail Han sheep. The result of genotyping showed that three genotypes were found at g.275981 C>T and g.276999 C>T loci in both year-round estrous and seasonal estrous sheep, both genotype frequency and allele frequency were very significantly different between year-round estrous and seasonal estrous sheep(P<0.01) in g.276999 C>T locus of MKRN3 gene. Population genetic analysis showed that the g.275981 C>T locus was at low polymorphism(PIC<0.25) in all sheep breeds; the g.276999 C>T locus was at moderate polymorphism(0.25T locus of Sunite and Cele Black sheep was under Hardy-Weinberg equilibrium(P>0.05); the g.276999 C>T locus of all sheep breeds was under HardyWeinberg equilibrium(P>0.05). Association analysis indicated that the polymorphism of g.275981 C>T and g.276999 C>T had no correlation with the litter size of the first, second or third parity in Small Tail Han sheep(P>0.05). Polymorphism of the 2 SNPs of MKRN3 gene had no significant effect on litter size of Small Tail Han sheep. |
| |
| Keywords: | Sheep MKRN3 gene SNP genotyping Litter size |
| 本文献已被 CNKI 等数据库收录！ |
|
