| 进口白羽肉种鸡禽白血病疫情鉴定 |
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| 引用本文： | 马美哥,于蒙蒙,许传田,黄庆华,孟照洁,王素艳,邢立晓,常芳芳,李猷,何希君,刘长军,祁小乐,王永强,孙延鸣,王笑梅,高玉龙.进口白羽肉种鸡禽白血病疫情鉴定[J].中国预防兽医学报,2019(5):526-529. |
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| 作者姓名： | 马美哥 于蒙蒙 许传田 黄庆华 孟照洁 王素艳 邢立晓 常芳芳 李猷 何希君 刘长军 祁小乐 王永强 孙延鸣 王笑梅 高玉龙 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽免疫抑制病创新团队;石河子大学动物科技学院;山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室;山东和康源生物育种股份有限公司 |
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| 基金项目： | 国家肉鸡产业技术体系(CARS-41-G15) |
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| 摘 要： | 2018年以来,某进口品种肉种鸡发生疑似禽白血病病例,流行范围广,危害严重,为确定该次疫情的病原,本研究从辽宁、山东等地发病鸡场采集55份疑似禽白血病病料样品。病理组织切片观察显示,病料样品肝脏和脾脏均有髓细胞样肿瘤细胞增生。针对禽白血病病毒(ALV)、鸡马立克氏病病毒(MDV)和网状内皮组织增生症病毒(REV)的特异性PCR检测结果显示,55份病料样品中ALV阳性样品12份(21.8%),MDV阳性样品1份(1.82%),REV均为阴性。将12份ALV阳性病料样品经处理后接种DF-1细胞进行病毒分离,ELISA检测结果显示有8份ALV群特异性抗原阳性。ALV多重PCR检测结果表明,8个分离株均为J亚群ALV(ALV-J),测序结果显示,8个分离株之间核苷酸序列同源性为99.5%~100%,与A、B、C、D、E亚群ALV的同源性仅为56.5%~58.8%,与ALV-J的同源性为90.7%~96.6%,进一步表明所有分离株均为ALV-J。上述结果表明,引起该次进口白羽父母代肉种鸡发生禽白血病的病原主要以ALV-J为主,该研究结果为疫情的溯源和有效防控奠定了基础。
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| 关 键 词： | 进口白羽肉种鸡 禽白血病 J亚群禽白血病病毒 病毒分离 |
Diagnosis of avian leukosis in imported broiler breeders |
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| MA Mei-ge,YU Meng-meng,XU Chuan-tian,HUANG Qing-hua,MENG Zhao-jie,WANG Su-yan,XING Li-xiao,CHANG Fang-fang,LI You,HE Xi-jun,LIU Chang-jun,QI Xiao-le,WANG Yong-qiang,SUN Yan-ming,WANG Xiao-mei,GAO Yu-long.Diagnosis of avian leukosis in imported broiler breeders[J].Chinese Journal of Preventive Veterinary Medicine,2019(5):526-529. |
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| Authors: | MA Mei-ge YU Meng-meng XU Chuan-tian HUANG Qing-hua MENG Zhao-jie WANG Su-yan XING Li-xiao CHANG Fang-fang LI You HE Xi-jun LIU Chang-jun QI Xiao-le WANG Yong-qiang SUN Yan-ming WANG Xiao-mei GAO Yu-long |
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| Institution: | (State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China;College of Animal Science and Technology,Shihezi University,Shihezi 832003,China;Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences,Shandong Key Lab of Animal Disease Control and Breeding,Jinan 250100,China;Biological Breeding Co.LTD of Shandong and Kangyuan,Jinan 271018,China) |
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| Abstract: | MA Mei-ge;YU Meng-meng;XU Chuan-tian;HUANG Qing-hua;MENG Zhao-jie;WANG Su-yan;XING Li-xiao;CHANG Fang-fang;LI You;HE Xi-jun;LIU Chang-jun;QI Xiao-le;WANG Yong-qiang;SUN Yan-ming;WANG Xiao-mei;GAO Yu-long(State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China;College of Animal Science and Technology,Shihezi University,Shihezi 832003,China;Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences,Shandong Key Lab of Animal Disease Control and Breeding,Jinan 250100,China;Biological Breeding Co.LTD of Shandong and Kangyuan,Jinan 271018,China) |
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| Keywords: | Imported broiler breeders avian leukosis subgroup J avian leukosis virus virus isolation |
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