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| 低温诱导甜菜抽薹基因的差异表达分析 | |
| 引用本文： | 戴建军,常缨,李彩凤,马凤鸣.低温诱导甜菜抽薹基因的差异表达分析[J].东北农业大学学报,2009,40(12). |
| 作者姓名： | 戴建军 常缨 李彩凤 马凤鸣 |
| 作者单位： | 1. 东北农业大学资源与环境学院,哈尔滨,150030 2. 东北农业大学生命科学学院,哈尔滨,150030 3. 东北农业大学农学院,哈尔滨,150030 |
| 基金项目： | 黑龙江省教委研究课题 |
| 摘 要： | 低温可以导致二年生糖用甜菜抽薹,使块根产量比正常甜菜产量减少10%～20%,含糖率降低0.8%～1.6%。采用代表性差异分析cDNA-RDA(Representational Difference Analysisofc DNA)技术,经过三轮差减杂交,获得在低温诱导甜菜茎尖中差异表达的基因片段DP3,为甜菜抽薹相关基因的进一步研究奠定了理论基础。 |
| 关 键 词： | 甜菜 低温诱导 抽薹 cDNA-RDA |
Differential expression analysis of bolting genes in the cold induced sugar bee |
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| DAI Jianjun,CHANG Ying,LI Caifeng,MA Fengming.Differential expression analysis of bolting genes in the cold induced sugar bee[J].Journal of Northeast Agricultural University,2009,40(12). | |
| Authors: | DAI Jianjun CHANG Ying LI Caifeng MA Fengming |
| Abstract: | Cold temperature that would induce a biannual sugar beet to bolting,which would lead the yield to decrease 10%-20%,and the sugar ratio to cut down 0.8%-1.6%.In this paper,(cDNA-RDA)was applied to clone genes specifically expressed in cold induced sugar beel.One cDNA fragment DP3 was obtained from shoot tips of cold induced sugar beet after three rounds of subtractive hybridization,which will supply a theoretical base for the further study of the bolting genes cloning from sugar beet. |
| Keywords: | cDNA-RDA sugar beet cold induced bolting representational difference analysis of cDNA |
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