| 金荞麦查尔酮合成酶基因CHS的克隆及序列分析 |
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| 引用本文： | 蒙华,李成磊,吴琦,邵继荣,陈惠.金荞麦查尔酮合成酶基因CHS的克隆及序列分析[J].草业学报,2010,19(3). |
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| 作者姓名： | 蒙华 李成磊 吴琦 邵继荣 陈惠 |
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| 作者单位： | 四川农业大学生命科学与理学院,四川,雅安,625014 |
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| 基金项目： | 四川省科技厅科技攻关项目 |
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| 摘 要： | 采用同源克隆的方法获得金荞麦查尔酮合成酶基因(CHS)的保守片段554 bp,进一步采用染色体步移法(genome-walking)和RT-PCR技术克隆到CHS基因的全长DNA序列和cDNA开放阅读框(ORF)序列.序列分析结果表明,金荞麦CHS基因DNA全长1 650 bp,含一个462 bp的内含子;其cDNA编码区全长1 188 bp,编码395个氨基酸,命名为FdCHS,NCBI登录号为GU169470.该氨基酸序列与同为蓼科的掌叶大黄、虎杖CHS的氨基酸序列同源率分别达到94%和93%,且含有CHS活性位点和底物结合口袋位点等保守位点.
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| 关 键 词： | 金荞麦 查尔酮合成酶基因 克隆 序列分析 |
Cloning and sequence analysis of the chalcone synthase gene (CHS) from Fagopyrum dibotrys |
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| MENG Hua,LI Cheng-lei,WU Qi,SHAO Ji-rong,CHEN Hui.Cloning and sequence analysis of the chalcone synthase gene (CHS) from Fagopyrum dibotrys[J].Acta Prataculturae Sinica,2010,19(3). |
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| Authors: | MENG Hua LI Cheng-lei WU Qi SHAO Ji-rong CHEN Hui |
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| Abstract: | The conservative sequence of the chalcone synthase gene from Fagopyrum dibotrys 544 bp was isolated by homology cloning,and then the full-length DNA and cDNA ORF (open reading frame) sequences were cloned by genome-walking and RT-PCR,respectively.Sequence analysis showed that the CHS DNA of F.dibotrys was 1 650 bp,including one 462 bp intron.The coding region of cDNA was 1 188 bp,encoding 395 amino acids,designated as FdCHS and included the conserved sites of CHS such as active sites and substrate-binding p... |
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| Keywords: | Fagopyrum dibotrys chalcone synthase gene cloning sequence analysis |
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