高压脉冲电场法提取耐辐射奇球菌中类胡萝卜素的研究

为探讨高压脉冲电场(PEF)电学参数致耐辐射奇球菌破壁提取类胡萝卜素的影响及最佳工艺参数,采用正交试验法,分析了电场强度、处理时间、频率和脉宽4种电场参数对类胡萝卜素提取效果的影响。结果表明,PEF技术提取类胡萝卜素的最优提取水平组合为:电场强度30 kV·cm^-1,处理时间30 s,频率30 Hz,脉宽10 μs。该条件下,提取量为75.06 μg·g^-1,与未进行电场处理的样品提取量48.27 μg·g^-1相比,增加了55.5%。本研究结果为PEF技术的工业化大规模应用提供了理论依据。

Study on the Extraction of Carotenoid From Deinococcus radiodurans Using High Voltage Pulsed Electric Field

To investigate the effect of high voltage pulsed electric field(PEF) on the extraction of carotenoid from deinococcus radiodurans, orthogonal test was applied to obtain the optimal process parameters. Four electric field parameters (electric field intensity, treatment time, frequency and pulse width)were optimized in this study, which were as follows: electric field intensity 30 kV·cm^-1, processing time 30 s, frequency 30 Hz, pulse width 10 μs. Under this operation condition, the extraction yield was to 75.06 μg·g^-1, which was 55.5% higher than the yield of control 48.27 μg·g^-1 (without electric field treatment). This study provide a theoretical guidance for the application of high voltage pulsed electric field technology on the extraction of carotenoid, which is capable for the large-scale industrial extraction.