谷子CIPK基因(Seita.5G145900)对非生物逆境的响应

在谷子基因组中鉴定出一个CIPK(Seita.5G145900,命名为SiCIPK19)基因。为揭示SiCIPK19对逆境胁迫的响应,对其基因结构、蛋白特征、功能、进化等性状进行了系统的分析和预测,并用实时定量PCR(RT-qPCR)检测了其在谷子苗期不同逆境及关键生育期干旱胁迫下的表达。结果表明,SiCIPK19基因位于谷子5号染色体,基因组序列长1 353 bp,编码450个氨基酸,基因无可变剪切,且不含内含子。功能域分析和多序列比对发现,SiCIPK19蛋白具有非常保守的序列结构,与其他植物CIPK蛋白也非常相似。RT-qPCR分析表明,SiCIPK19基因被聚乙二醇6000(PEG 6000)、ABA、高盐和低温胁迫强烈诱导。此外,SiCIPK19基因在谷子拔节期、抽穗期和灌浆期干旱条件下参与了对干旱胁迫的响应,推测该基因参与谷子对非生物逆境的应答,尤其在抽穗期和灌浆期干旱胁迫应答中发挥重要作用。本研究结果为进一步分析CIPK基因逆境应答机制,以及利用基因工程方法改善谷子抗逆性和提高产量提供了理论支持。

Response to Abiotic Stresses of SiCIPK Gene (Seita.5G145900) in Foxtail Millet

A SiCIPK gene (Seita.5G145900, named as SiCIPK19 temporarily) was identified in foxtail millet genome. In order to reveal the response to abiotic stress, the gene structure, protein characteristics, function, evolution and other traits of SiCIPK19 were analyzed and predicted. The real-time quantitative PCR (RT-qPCR) was used to detect the induced expression of SiCIPK19 under different stresses during seedling stage and by drought during key growth stages. The results showed that SiCIPK19 is located on chromosome 5 in foxtail millet genome. SiCIPK19 has a length of 1 353 bp, encodes 450 amino acids residues. The gene has no alternative splicing and intron. Functional domain analysis and multiple sequence alignment revealed that the SiCIPK19 protein has an extreme conserved sequence structure and is very similar to other plant CIPK proteins. RT-PCR analysis showed that the expression of SiCIPK19 was strongly up-regulated under PEG, ABA, salt and low temperature. Further studies indicated that SiCIPK19 was involved in drought stress in the jointing, booting and filling stage. It is speculated that SiCIPK19 is involved in the response to abiotic stress in foxtail millet, plays an important role at the booting stage and filling stage under drought stress. This study provided experimental date and theoretical support for further analysis on responing mechanism of CIPK to stress and improving crop stress resistance and yield using genetic engineering methods.