低温胁迫对高羊茅生长影响的研究

以高羊茅Festuca arundinacea草坪草为材料,设置3种低温处理:-6、5和0 ℃处理8 h,并以室温为对照，然后测定过氧化氢酶（CAT）活性、电解质渗透率、可溶性糖含量、脯氨酸含量和叶绿素含量等生理指标。结果表明,在5 ℃下, CAT的活性在胁迫4 h时活性最大；当温度为0与-6 ℃时,酶的活性随着胁迫时间的延长而急剧下降,温度越低,下降越快。叶绿素含量随着胁迫时间的延长而下降,温度越低下降越快。脯氨酸含量在0与-6 ℃时,随着时间的延长,含量则明显增加。在-6 ℃时, 电解质渗透率明显增大。可溶性糖含量随胁迫时间延长而上升，-6 ℃时，可溶性糖含量明显上升，低温抗寒锻炼能有效增强高羊茅的抗寒性。

The effect of low temperature stress on tall fescue growth

Tall fescue was treated at low temperature (-6 ℃, 5 ℃ and 0 ℃) for eight hours. The room temperature was used as control, physiological indexes such as catalase activity, leakage rate of electrolyte, the content of soluble sugar, proline and chlorophyll were measured. The results showed that the activity of CAT reached the peak after 4 hours stress at 5 ℃, the enzyme activity and the content of chlorophyll decreased sharply with increasing stress time under 0 ℃ and -6 ℃. Enzyme activity under -6 ℃ decreased faster than that under 0 ℃. The content of proline increased significantly with the increasing stress time under 5 ℃ and 0 ℃. leakage rate of electrolyte increased significantly at -6 ℃. Soluble sugar content increased with the increasing stress time, it increased markedly at -6 ℃. Low temperature can effectively enhance the cold resistance of tall fescue.