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| 哈密瓜ACC氧化酶cDNA克隆及其序列分析 | |
| 引用本文： | 陆璐,王鸣,郑学勤,李小娟. 哈密瓜ACC氧化酶cDNA克隆及其序列分析[J]. 园艺学报, 2000, 27(1): 32-36 |
| 作者姓名： | 陆璐 王鸣 郑学勤 李小娟 |
| 作者单位： | 1. 甘肃农业大学园艺系,兰州,730070 2. 西北农业大学园艺系,杨凌,712100 3. 中国热带农业科学院热带作物生物技术国家重点实验室,海口,571101 |
| 基金项目： | 实验室开放研究基金资助 |
| 摘 要： | 以哈密瓜成熟果实为材料，用改进的异硫氰酸胍一步法提取总ＲＮＡ，通过ＲＴ－ＰＣＲ方法和ＤＮＡ序列测定证实获得了甜瓜ＡＣＣ氧化酶多基因家族中一个与果实后熟有关的基因ＣＭ－ＡＣＯ１的ｃＤＮＡ克隆ｐＣＭ－ＡＣ０１，序列分析结果表明该序列与国外以罗马甜瓜为材料获得的相同基因的ｃＤＮＡ序列完全相符。推断该基因在甜瓜种内可能是完全或高度保守的，不同类群之间无进化上的分歧。 |
| 关 键 词： | 哈密瓜 ACC氧化酶 序列分析 CM－AC01 耐贮运性 |
| 修稿时间： | 1999-05-04 |
Cloning and Sequence Analysis of a cDNA Encoding ACC Oxidase from Hamimelon Fruit |
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| Lu Lu,Wang Ming,Zheng Xueqin,Li Xiaojuan. Cloning and Sequence Analysis of a cDNA Encoding ACC Oxidase from Hamimelon Fruit[J]. Acta Horticulturae Sinica, 2000, 27(1): 32-36 | |
| Authors: | Lu Lu Wang Ming Zheng Xueqin Li Xiaojuan |
| Abstract: | Total RNA was extracted from mature fruits of Hamimelon( Cucumis melo L.).RT PCR analysis using specific primers was used to amplified target fragment.A cDNA clone pCM ACO1 encoding a 1 Aminocyclopropane 1 Carboxylic acid Oxidase homology(ACO1)which responsible for melon fruit ripening has been obtained.The sequence of this cDNA contains 1236bp and coding region has 957bp encoding 318 predicted amino acid residues.Comparison with the first ACO1 cDNA pCM ACO1 which was identified in cantaloupe( C.melo var. cantaloupensis Naud.)shows the complete conformity.We give the results that the ACO1 gene among the species of C.melo is highly conserved,there may be no evolution divergences among different varieties. |
| Keywords: | Melon ACC oxidase RT PCR Sequence analysis |
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