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| Na+,K+-ATPase调节肝再生增强因子促HepG2细胞增殖 | |
| 引用本文： | 林莹,佟明华,汪嵘,孔祥平,梁世中. Na+,K+-ATPase调节肝再生增强因子促HepG2细胞增殖[J]. 广西农业生物科学, 2006, 25(1): 1-5 |
| 作者姓名： | 林莹 佟明华 汪嵘 孔祥平 梁世中 |
| 作者单位： | 1. 华南理工大学,生物科学与工程学院,广东,广州,510640;广西大学,生命科学与技术学院,广西,南宁,530005 2. 解放军第458医院,全军传染病中心,广东,广州,510600 3. 广西大学,生命科学与技术学院,广西,南宁,530005 4. 华南理工大学,生物科学与工程学院,广东,广州,510640 |
| 基金项目： | 广东省自然科学基金资助项目(04102063) |
| 摘 要： | 采用四甲基噻唑盐(M TT)法检验肝再生增强因子(ALR)对H epG 2细胞增殖作用;[3H]-T dR掺入测定细胞DNA合成;采用无机磷比色法测定细胞N a ,K -ATPase的酶活力。结果表明:ALR通过促进H epG 2细胞DNA合成,使细胞增殖,并存在剂量效应正相关性(P<0.01);ALR对N a ,K -ATPase酶活呈剂量时间依赖型影响;奎巴因可以通过抑制细胞N a ,K -ATPase影响ALR对H epG 2细胞增殖促进作用。因此,N a ,K -ATPase能参与调节ALR促进H epG 2细胞的增殖。 |
| 关 键 词： | 肝再生增强因子(ALR) Na K -ATPase 酶活力 增殖 |
| 文章编号： | 1008-3464(2006)01-0001-05 |
| 修稿时间： | 2005-10-09 |
Na+, K+-ATPase modulate the proliferation of HepG2 cell caused by augmenter of liver regeneration |
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| LIN Ying,TONG Ming-hua,WANG Rong,KONG Xiang-ping,LIANG Shi-zhong. Na+, K+-ATPase modulate the proliferation of HepG2 cell caused by augmenter of liver regeneration[J]. Journal of Guangxi Agricultural and Biological Science, 2006, 25(1): 1-5 | |
| Authors: | LIN Ying TONG Ming-hua WANG Rong KONG Xiang-ping LIANG Shi-zhong |
| Abstract: | MTT assay and -TdR incorporation were used to test the proliferation of HepG2 cell caused by augmenter of liver regeneration(ALR).Inorganic phosphorus spectrophotometer was used to test the activities of the Na~ ,K~ ATPase.The results showed that ALR could promote the proliferation of HepG2 cell by increasing DNA synthesize rate.Na~ ,K~ -ATPase activity was stimulated by ALR which depended upon the time and concentration.The proliferation effect which was caused by ALR were inhibited by ouabain,the Na~ ,K~ -ATPase inhibitor.Na~ ,K~ -ATPase contributed the regulation of ALR in proliferation of HepG2 cell. |
| Keywords: | augmenter of liver regeneration(ALR) Na~ K~ -ATPase enzyme activity proliferation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |