Simultaneous determination of vitamin B1 and B2 in complex cereal foods,by reverse phase isocratic HPLC-UV

The evaluation of nutritional or functional components in grain products is an important feature for the industry, especially when recent regulations require a correct nutrition labelling, valid during all the shelf life of the product. For that reason, industry usually makes many efforts to develop simple and reliable analytical methods that can be easily applied in any quality control laboratories for routine analysis. Spectrofluorimetric analysis of thiamine and riboflavin are sensitive, but need specific equipment. A few HPLC-UV methods have been described but they are less sensitive, and present difficulties due to interfering compounds, particularly in complex food matrixes, as grains and derivatives.