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106份狗牙根材料的SRAP分析
引用本文:张延辉,李江华,孙强,帕提古丽,阿不来提.106份狗牙根材料的SRAP分析[J].草原与草坪,2013,33(2):1-10.
作者姓名:张延辉  李江华  孙强  帕提古丽  阿不来提
作者单位:1. 新疆农业大学草业与环境科学学院/新疆草地资源与生态重点实验室,新疆乌鲁木齐,830052
2. 新疆维吾尔自治区乌鲁木齐市公安局,新疆乌鲁木齐,830052
3. 新疆维吾尔自治区草原总站,新疆乌鲁木齐,830052
基金项目:国家自然基金,新疆农业大学国家重点学科项目XJCYB-2011-05资助
摘    要:对采自新疆不同地域及引自国内外其他地区的106份狗牙根材料开展SRAP标记研究。结果表明:14对SRAP引物共扩增产生133条带,其中,123条显示多态性,多态位点百分率为92.5%;106份狗牙根材料的GS值在0.36~0.94,平均为0.65,各供试材料之间差异明显,具有较为丰富的遗传多样性;聚类分析表明,106份供试材料相似系数为0.50时可被聚为3类,聚类结果与地理来源有一定的相关性。

关 键 词:狗牙根  遗传多样性  SRAP

SRAP analysis of 106 Cynodon dactylon lines
ZHANG Yan-hui , LI Jiang-hua , SUN Qiang , PATIGULI , ABULAIT.SRAP analysis of 106 Cynodon dactylon lines[J].Grassland and Turf,2013,33(2):1-10.
Authors:ZHANG Yan-hui  LI Jiang-hua  SUN Qiang  PATIGULI  ABULAIT
Institution:ZHANG Yan-hui1,LI Jiang-hua2,SUN Qiang3,PATIGULI1,ABULAIT(1.Xiniang Key Laboratory of Grassland Resources and Ecology,Department of Grassland and Environment Science,Xinjiang Agriculture University,Urumqi 830052,China;2.Public Security Bureau of Urumqi, Urumqi 830052,China;3.Grasslands Station of Xinjiang,Urumqi 830052,China)
Abstract:The genetic diversity of Cynodon dactylon from Xinjiang was investigated by using 106 materials through SRAP marker.133 bands were amplified by 14 pairs of informative and reliable primers,of which 123 were polymorphic,the percentage of polymorphic sites was 92.5%;Genetic similarity(GS) of 48 materials ranges from 0.36 to 0.94,the average was 0.65.The variation among all materials was obvious,which showed that the genetic diversity was high.This data illustrated that the genetic variation within population was larger than among populations.The general clustering consequences indicated that 106 materials were classified into three groups when the similarity coefficient was 0.50,the classification results was correlated with geographical distribution,but some materials were except.
Keywords:Cynodon dactylon  genetic diversity  SRAP
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