小麦抗叶锈基因Lr45的SCAR标记

 【目的】建立小麦抗叶锈基因Lr45 SCAR（sequenced characterized amplified region序列特征扩增区域）标记。【方法】以小麦抗叶锈病基因材料TcLr45和感病材料Thatcher为亲本，利用黑麦基因组特异的RAPD标记引物OPH20进行PCR扩增，对获得的与预期大小相同的1.5kb的特异片段进行克隆测序，并根据该序列设计一对特异PCR引物LRYR和LRYF，对TcLr45×Thatcher F2代单株构建的分离群体进行扩增，验证该标记与Lr45的连锁关系，Mapmaker 3.0软件绘制遗传连锁图。【结果】该标记在TcLr45中扩增出单一条带，片段大小为1 272 bp（命名为Ypsc20H1272），而在感病亲本中则无扩增条带。F2代分离群体进行连锁分析，遗传距离为8.2 cM，该标记为与Lr45连锁的SCAR标记。【结论】将Lr45的RAPD标记转化成SCAR标记。

A SCAR Marker for Leaf Rust Resistance Gene Lr45

【Objective】 The objective was to develop a SCAR(sequenced characterized amplified region) marker for wheat leaf rust resistance gene Lr45. 【Method】 Wheat leaf rust resistance near isogenic lines of TcLr45 and its susceptible control Thatcher were employed. PCR was carried out with the OPH20, which is a specific RAPD primer of rye. The amplified 1.5kb fragment from TcLr45 was cloned and sequenced. Subsequently a pair of specific primers (LRYR, LRYF) were designed for the amplifying F2 population derived from TcLr45×Thatcher, and then linkage was analyzed between the marker and Lr45. 【Result】 A unique band was obtained only in TcLr45 with a length of 1 272 bp. And the marker was named as Ypsc20H1272. The linkage distance of the SCAR marker with Lr45 was 8.2 cM.【Conclusion】 The RAPD marker was successfully converted into a SCAR marker for Lr45.