| 土田七幼嫩根茎的组培快繁 |
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| 引用本文： | 李冬梅,赵超艺,刘小飞.土田七幼嫩根茎的组培快繁[J].热带农业科学,2017(10):45-49. |
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| 作者姓名： | 李冬梅 赵超艺 刘小飞 |
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| 作者单位： | 广东省农业科学院环境园艺研究所 广东广州510640 |
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| 基金项目： | 广东省科技计划项目(2015A020209078、2015B070701016)，广州市科技计划项目(201607010101) |
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| 摘 要： | 以土田七的幼嫩根茎为外植体,通过对其芽启动诱导、继代增殖及生根诱导培养基的筛选,建立了土田七幼嫩根茎的离体快繁体系。结果表明:土田七幼嫩根茎的最佳芽诱导培养基为MS+6-BA 3.0 mg/L+NAA0.10 mg/L+3%蔗糖+卡拉胶粉9.5 g/L+10%椰汁;继代增殖最佳的培养基为MS+6-BA 5.0 mg/L+NAA 0.10 mg/L+3%蔗糖+卡拉胶粉9.5 g/L+10%椰汁;生根最佳的培养基为MS+NAA 0.10 mg/L+3%蔗糖+卡拉胶粉9.5 g/L+10%香蕉泥+活性碳1.0 g/L;组培苗移栽较合适的基质为进口泥炭∶红壤∶腐叶土混合的基质。
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| 关 键 词： | 土田七 土田七属 根茎 繁殖 |
Rapid Propagation of Stahlianthus involucratus via in Vitro Culture of Young Rhizomes |
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| LI Dongmei,ZHAO Chaoyi,LIU Xiaofei.Rapid Propagation of Stahlianthus involucratus via in Vitro Culture of Young Rhizomes[J].Chinese Journal of Tropical Agriculture,2017(10):45-49. |
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| Authors: | LI Dongmei ZHAO Chaoyi LIU Xiaofei |
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| Abstract: | Young rhizomes of Stahlianthus involucratus were used as explants for tissue culture.Media for bud inducing, subculture and rooting were screened to establish a rapid propagation system based on young rhizomes of S. involucratus. The results showed that the optimum medium was MS+6-BA 3.0 mg/L+NAA 0.10 mg/L+3% sucrose+carrageenan powder 9.5 g/L+10% coconut water for inducing of adventitious buds, MS+6-BA 5.0 mg/L+NAA 0.10 mg/L+3% sucrose+carrageenan powder 9.5 g/L+10% coconut water for subculture, and MS+NAA 0.10 mg/L+3% sucrose+carrageenan powder 9.5 g/L+10% mashed banana + activated carbon 1.0 g/L for rooting. The optimum medium for transplanting of the rooted plantlets was the mixture of imported peat,red soil and humus soil. |
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| Keywords: | Stahlianthus involucratus Stahlianthus rhizome propagation |
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