Detection of Plasmodiophora Brassicae By PCR in Naturally Infested Soils |
| |
Authors: | Ann-Charlotte Wallenhammar Ola Arwidsson |
| |
Institution: | (1) Plant Pathology and Biocontrol Unit, SLU, P.O. 7035, SE-750 07 Uppsala, Sweden;(2) Swedish Federation of Rural Economy and Agricultural Societies, P.O. 271, SE 701 45 Örebro, Sweden |
| |
Abstract: | A nested polymerase chain reaction (PCR) method was developed for detection of DNA from Plasmodiophora brassicae in naturally infested field soil samples. The target sequences 389 bp and 507 bp were amplified from Swedish populations of P. brassicae. The protocols described enabled detection of DNA in various soil classes with an inoculum level of P. brassicae corresponding to a disease severity index (DSI) higher than 21 in a greenhouse bioassay. Three sequenced Swedish P. brassicae isolates had identical sequence in the 18S/ITS 1 region, but differed by a few nucleotides from an isolate sequenced in the UK. The results indicate that the primers used are general for P. brassicae, and consequently the nested PCR assay has a potential to be developed as a routine diagnostic test. |
| |
Keywords: | DNA-detection nested-PCR sequencing soil extraction |
本文献已被 SpringerLink 等数据库收录! |
|