| 家蚕蚕卵超氧化物歧化酶初步研究 |
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| 引用本文： | 魏国清,代君君,田恒杰,王为联.家蚕蚕卵超氧化物歧化酶初步研究[J].安徽农业大学学报,2004,31(2):194-196. |
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| 作者姓名： | 魏国清 代君君 田恒杰 王为联 |
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| 作者单位： | 1. 安徽农业大学蚕业丝绸系,合肥,230036
2. 安徽省灵璧县农业局,灵璧,234200 |
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| 基金项目： | 安徽省自然科学基金
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安徽省高校青年教师科研项目 |
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| 摘 要： | 用聚丙烯酰胺凝胶电泳研究蚕卵超氧化物歧化酶(SOD)同工酶的初步性质,利用SOD抑制氮蓝四唑(NBT)光还原反应测定了蚕卵SOD酶学活力,并研究了不同抑制剂对蚕卵SOD活性的影响.结果表明,4个供试蚕品种蚕卵SOD同工酶都有1条主带;蚕卵中SOD同工酶有Mn-SOD、CuZn-SOD 2种类型,以Mn-SOD为主,CuZn-SOD次之;不同品种之间蚕卵SOD酶学活力相近,同工酶主带一致,副带略有差异.
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| 关 键 词： | 蚕卵 超氧化物歧化酶 抑制剂 |
| 文章编号： | 1672-352X(2004)02-0194-03 |
| 修稿时间： | 2003年8月26日 |
Preliminary Study on Superoxide Dismutase in Eggs of Silkworm |
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| WEI Guo-qing,DAI Jun-jun,TIAN Heng-jie,WANG Wei-lian.Preliminary Study on Superoxide Dismutase in Eggs of Silkworm[J].Journal of Anhui Agricultural University,2004,31(2):194-196. |
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| Authors: | WEI Guo-qing~ DAI Jun-jun~ TIAN Heng-jie~ WANG Wei-lian~ |
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| Institution: | WEI Guo-qing~1,DAI Jun-jun~1,TIAN Heng-jie~2,WANG Wei-lian~2 |
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| Abstract: | The activity of superoxide dismutase in silkworm eggs was determined by means of polyacrylamide gel electrophoresis and NBT light reducing reaction.The results indicated that the SOD isozyme exhibited a main bright superoxide dismutase band and several dark bands in the gel.Its activity could be strongly inhibited by chloroform-ethanol and slightly inhibited by H_2O_2.So it could be considered that the Mn-SOD exhibited the main activity of superoxide dismutase in the silkworm eggs. |
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| Keywords: | silkworm eggs superoxide dismutase inhibitor |
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