| 药用唇柱苣苔叶片分化及植株再生研究 |
| |
| 引用本文： | 姚绍嫦,凌征柱,李翠,吕惠珍,张占江.药用唇柱苣苔叶片分化及植株再生研究[J].种子,2011,30(12). |
| |
| 作者姓名： | 姚绍嫦 凌征柱 李翠 吕惠珍 张占江 |
| |
| 作者单位： | 广西壮族自治区药用植物园,广西药用资源保护与遗传改良重点实验室, 南宁530023 |
| |
| 基金项目： | 广西科学研究与技术开发计划项目(No.0895001-3-2); 广西卫生厅科研课题(Z 2009345) |
| |
| 摘 要： | 目的:建立濒危药用植物药用唇柱苣苔叶片分化及植株再生体系.方法:以药用唇柱苣苔(Chirita medica D.Fang ex W.T.Wang)叶片为外植体,灭菌后接种到附加不同激素配比的培养基中培养,筛选各阶段合适的培养基.结果:不定芽诱导的最适培养基为MS+ 1.0 mg/L 6-BA+ 0.2 mg/L NAA,pH =6.0,诱导率为100％；最佳继代增殖培养基为MS+0.5 mg/L 6-BA+ 0.2 mg/L NAA,pH6.0,增殖系数为12.6/30 d；最适合的生根培养基为1/2 MS+ 0.2 mg/L NAA,pH=6.0,生根率高达100％,移栽成活率为95.0％.结论:本繁殖体系可在短时间内提供大量种苗,并为种质资源的保护和可持续利用提供技术指导.
|
| 关 键 词： | 药用唇柱苣苔 分化 植株再生 |
Research on Differentiation and Plant Regeneration From in Vitro Leaf Tissues of Chirita medica D.Fang ex W.T.Wang |
| |
| YAO Shao-chang
,
LING Zheng-zhu
,
LI Cui
,
LU Hui-zhen
,
ZHANG Zhan-jiang.Research on Differentiation and Plant Regeneration From in Vitro Leaf Tissues of Chirita medica D.Fang ex W.T.Wang[J].Seed,2011,30(12). |
| |
| Authors: | YAO Shao-chang LING Zheng-zhu LI Cui LU Hui-zhen ZHANG Zhan-jiang |
| |
| Institution: | YAO Shao-chang,LING Zheng-zhu,LI Cui,LU Hui-zhen,ZHANG Zhan-jiang(Guangxi Botanical Garden of Medicinal Plant,Guangxi key Laboratory of medicinal Resources conservation and Genetic Improvement,Nanning 530023,China) |
| |
| Abstract: | Objective:To develop differentiation and plant regeneration system from in vitro leaf tissues of Chirita medica D.Fang ex W.T.Wang,an endangered medicinal species.Methods:The leaf of Chirita medica as explants were cultured on MS supplemented with different kinds and concentrations of hormone to select optimized different phase.Results:The optimum medium for adventitious buds induction and seedling regeneration was MS+1.0 mg/L 6-BA+0.2 mg/L NAA(pH 6.0) and the induce rate of bud regeneration was 100%.The ef... |
| |
| Keywords: | Chirita medica D Fang ex W T Wang differentiation plant regeneration |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
