| 食品中产单核细胞李斯特菌PCR检测方法的建立 |
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| 引用本文： | 白艳艳,张彦明,郑增忍,张衍海,龚振华,张宝.食品中产单核细胞李斯特菌PCR检测方法的建立[J].中国动物检疫,2008,25(1):29-30,45. |
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| 作者姓名： | 白艳艳 张彦明 郑增忍 张衍海 龚振华 张宝 |
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| 作者单位： | 1. 西北农林科技大学动物医学院,陕西杨陵,712100
2. 中国动物卫生与流行病学中心,266032 |
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| 基金项目： | 陕西省重大科技专项（2006kz07-G2） |
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| 摘 要： | 根据产单核细胞李斯特菌hlyA基因设计引物,进行PCR扩增,检测该方法的特异性和灵敏度。人工污染样品经Half-fraser和Fraser增菌后进行PCR检测。结果表明,产单核细胞李斯特菌扩增出234bp的条带,对照菌未扩增出目的条带。该方法的灵敏度为104cfu/mL。人工污染样品的检出限为8cfu/25g,说明PCR方法检测食品中产单核细胞李斯特菌具有快速、特异、敏感等特点,具有较高的实用价值。
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| 关 键 词： | 产单核细胞李斯特菌 PCR 检测 |
Establishment of PCR detection Method for Listeria monocytogenes in Food |
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| BAI Yanyan,ZHANG Yanming,ZHENG Zengren,ZHANG Yanhai,GONG Zhenhu,ZHANG Bao.Establishment of PCR detection Method for Listeria monocytogenes in Food[J].China Journal Of Animal Quarantine,2008,25(1):29-30,45. |
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| Authors: | BAI Yanyan ZHANG Yanming ZHENG Zengren ZHANG Yanhai GONG Zhenhu ZHANG Bao |
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| Institution: | 1) (1.College of Animal Science and Technology, Northwest A& F University, Yangling, Shaanxi 712100, China; 2. Animal health and Epidemiology Center of China, Qingdao, Shandong 266032 ,China ) |
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| Abstract: | A PCR was established for the detection of Listeria monoeytogenes (LM) in food with a pair of primers specific to the LM hly A gene. The sensitivity and specificity of this method were detected. The procedure was validated by detecting Listeria monocytogenes in artificially contaminated food and PCR analysis was taken following Half- fraser and fraser enrichment steps. The specific 234 bp fragment was amplified in LM while none was ampli- fied in all other species. The specificity is 104 cfu /mL.As few as 8 cfu /25 g can be detected in artificially con- taminated food. The PCR procedure has proved to be a rapid sensitive and specific method suitable for detecting Listeria monocytogenes in food. |
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| Keywords: | Listeria monocytogenes PCR detection |
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