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| 人工养殖大鲵病原荧光假单胞菌的分离鉴定 | |
| 引用本文： | 杜宗君,黄小丽,邓永强,汪开毓,耿毅,陈德芳. 人工养殖大鲵病原荧光假单胞菌的分离鉴定[J]. 四川农业大学学报, 2011, 29(1): 103-107. DOI: 10.3969/j.issn.1000-2650.2011.01.020 |
| 作者姓名： | 杜宗君 黄小丽 邓永强 汪开毓 耿毅 陈德芳 |
| 作者单位： | 1. 四川农业大学动物科技学院水产系,四川雅安,625014 2. 四川省动物疫病预防控制中心,成都,614000 3. 四川农业大学鱼病研究中心,四川雅安,625014 |
| 基金项目： | 中央级农业公益性行业科研专项经费项目，教育部"长江学者和创新团队发展计划"创新团队项目 |
| 摘 要： | 从四川洪雅人工养殖的患病大鲵(Andrias davidianus)肝、肾中分离到一株优势菌(S100814),以腹腔注射、浸泡的方式进行人工感染试验,证实其为养殖大鲵的病原菌.该分离株生理生化特性与荧光假单胞菌一致,该菌为革兰氏阴性短杆菌,在TSA平板上生长呈边缘整齐、半透明的圆形灰白色菌落.接触酶、精氨酸双水解酶、... |
| 关 键 词： | 大鲵 荧光假单胞菌 分离 鉴定 |
Isolation and Identification of Pseudomonas fluorescents from Andrias davidianus |
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| DU Zong-jun,HUANG Xiao-li,DENG Yong-qiang,WANG Kai-yu,GENG Yi,CHEN De-fang. Isolation and Identification of Pseudomonas fluorescents from Andrias davidianus[J]. Journal of Sichuan Agricultural University, 2011, 29(1): 103-107. DOI: 10.3969/j.issn.1000-2650.2011.01.020 | |
| Authors: | DU Zong-jun HUANG Xiao-li DENG Yong-qiang WANG Kai-yu GENG Yi CHEN De-fang |
| Abstract: | Bacterial strain S100814 was isolated from cultured Andrias davidianus in Hongya Sichuan Province.The strain was proved to be the pathogen of the disease by artificial infection test.The isolated strain was identified as Pseudomonas fluorescents by morphology,physiology,as well as 16SrDNA sequence(Accessible Number in GenBank:HQ880245).The isolated strain in TSA was hoar circular translucent colonies.The stain was short gram-negative bacterium.Glucose,arabinose,sorbitol,inositol,contact enzyme,two-arginine hydrolase,liquefied gelatin were positive;Maltose,lactose,mannitol,indole reaction,V-P test,maltose,H2S production were negative.It's physiological and biochemical characteristics were same with Pseudomonas fluorescence.The results showed that the strain was sensitive to doxycycline and novobiocin and resistance to enrofloxacin,gentamycin,amikacin,and florfenicol. |
| Keywords: | Andrias davidianus Pseudomonas fluorescents isolation identification |
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