山梨醇作碳源对苹果微体繁殖的效应

比较山梨醇和蔗糖两种碳源在苹果微体繁殖中的作用表明，二者对增殖和生长的最适浓度均为３０ｇ／Ｌ．山梨醇代替蔗糖作碳源时芽的增殖倍数高，而新梢生长差，叶／茎值小。总碳源为３０ｇ／Ｌ时，采用不同比例的山梨醇和蔗糖配合有利于芽的增殖和生长。山梨醇对生根的作用显著低于蔗糖，而在山梨醇培养基上继代４次后，生根得到明显改善

Effect of Sorbitol as Carbon Source on Micropropagation of Apple

The influences of two carbon source,sorbitol and sucrose,in micropropa-gation of apple were compared.The results showed that the optimal concentration ofboth sorbitol and sucrose was 30 g/L for shoot proliferation and growth.The substitu-tion of sorbitol for sucrose could promote proliferation,but could decrease shoot growthleaf/stem ratio,A medium with various proportion with total carbon source of 30 g/L ofsorbitol and suerose favored both proliferation and shoot growth.Rooting was worse onsorbitol medium than on sucrose one but would be greatly enhanced after subcultured 4times.