|
|||||
|
|
| 鸭瘟病毒转移质粒的构建 | |
| 引用本文： | 张馨月,冉多良,叶伟成,袁秀芳,王一成,刘蔓雯,张存.鸭瘟病毒转移质粒的构建[J].动物医学进展,2010,31(3):25-29. |
| 作者姓名： | 张馨月 冉多良 叶伟成 袁秀芳 王一成 刘蔓雯 张存 |
| 作者单位： | 1. 浙江省农科院畜牧兽医研究所,浙江杭州,310021;新疆农业大学动物医学学院,新疆乌鲁木齐,830052 2. 新疆农业大学动物医学学院,新疆乌鲁木齐,830052 3. 浙江省农科院畜牧兽医研究所,浙江杭州,310021 |
| 基金项目： | 浙江省自然科学基金项目(Y306179) |
| 摘 要： | 为了建立重组鸭瘟病毒技术,构建了鸭瘟病毒转移质粒。在对鸭瘟强毒和弱毒株TK基因进行测序分析后,将鸭瘟病毒TK-UL24DNA片段克隆于pUC18载体中,构建了质粒pTK;将PCR扩增的GFP真核表达盒插入pTK质粒的TK基因内部,获得转移载体质粒pTK-GFP。鸭瘟病毒TK-UL24测序分析表明鸭瘟强、弱毒株TK基因序列完全相同;转移载体携带Pcmv-GFP-SV40pA表达盒,测序验证其序列与源序列一致。pTK-GFP在脂质体介导下,转染鸭胚成纤维细胞和鸭肾细胞,在荧光显微镜下观察绿色荧光蛋白表达情况。质粒转染细胞后,绿色荧光蛋白得到了有效的表达,为进一步开展重组鸭瘟病毒的研究和构建具有遗传标记的鸭瘟疫苗奠定了基础。 |
| 关 键 词： | 鸭瘟病毒 TK基因 转移载体 |
Construction of Transfer Plasmid for Duck Enteritis Virus |
|
| ZHANG Xin-yue,RAN Duo-liang,YE Wei-cheng,YUAN Xiu-fang,WANG Yi-cheng,LIU Man-wen,ZHANG Cun.Construction of Transfer Plasmid for Duck Enteritis Virus[J].Progress In Veterinary Medicine,2010,31(3):25-29. | |
| Authors: | ZHANG Xin-yue RAN Duo-liang YE Wei-cheng YUAN Xiu-fang WANG Yi-cheng LIU Man-wen ZHANG Cun |
| Institution: | 1.Institute of Animal Husbandry and Veterinary Medicine;Zhejiang Academy of Agricultural Sciences;Hangzhou;Zhejiang;310021;China;2.College of Veterinary Medicine;Xinjiang Agricultural University;Urmuqi;Xinjiang;830052;China |
| Abstract: | A transfer plasmid was constructed for the recombinant of duck enteritis virus(DEV).Based on analysing of TK-UL24 DNA sequences of virulent and vaccine DEV strains,the TK-UL24 DNA segment from DEV was cloned into pUC18 to generate the pTK plasmid;An eukaryotic expression cassette containing the green fluorescene protein(GFP)gene was amplified by PCR and inserted into the TK gene in pTK plasmid to produce transfer vector pTK-GFP.The seqencing results showed that TK-UL24 sequence of the vaccine virus was iden... |
| Keywords: | Duck enteritis virus TK gene transfer plasmid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |