| 树莓组织培养最佳外植体材料试验 |
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| 引用本文： | 王岳英.树莓组织培养最佳外植体材料试验[J].山西林业科技,2009,38(2):12-13,30. |
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| 作者姓名： | 王岳英 |
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| 作者单位： | 山西省桑干河杨树丰产林实验局,山西,大同,037006 |
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| 摘 要： | 以树莓一年中不同时期的材料及同一植株不同部位的器官作外植体进行试验。结果表明：4月份采集的外植体污染率最低,成活率最高,即4月份是树莓组织培养最佳取材时期;在初代培养中,茎段的诱导率最高,为79.3%,带芽茎段是树莓组织培养最佳外植体材料;0.5%NaClO消毒20 min,是树莓组织培养外植体的最佳消毒方法。
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| 关 键 词： | 树莓 组织培养 外植体 |
Selection of Optimal Explant of Raspberry |
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| Wang Yueying.Selection of Optimal Explant of Raspberry[J].Shanxi Forestry Science and Technology,2009,38(2):12-13,30. |
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| Authors: | Wang Yueying |
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| Institution: | Wang Yueying (Sangganhe Poplar High Yield Forest Bureau of Shanxi, 037006 Datong, China) |
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| Abstract: | Explants of raspberry were selected from different tissue parts at different times during a year. The experiment showed that： The explant taken in April showed a relatively high survival rate and low contamination percentage. So April is the best time to take explant. Induction rate of stem segment is the highest at initial culture, reaches by 79.3%. The stem segment with buds was the optimal explant. The better sterilization method is 0.5 % of NaCIO solution about 20 minutes. |
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| Keywords: | raspberry tissue culture explant |
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