奶水牛乳汁体细胞数与抗氧化指标的相关性分析

目的]了解奶水牛乳汁体细胞计数（SCC）与抗氧化指标之间的相关性,为乳汁质量监控和奶水牛健康养殖提供科学依据.方法]随机选取南宁某牛场奶水牛139头,同步采集乳样与血样,乳汁SCC采用FOSS体细胞仪测定,血浆和乳清的总抗氧化能力（TAC）采用FRAP法（血浆铁还原力法）测定,丙二醛（MDA）含量采用TBA法（硫酸巴比妥法）测定.结果]被检的139头奶水牛中,乳汁SCC≥50.00万/mL（隐性乳房炎诊断标准）的样本占被检总数的12.23％,乳汁SCC＜50.00万/mL的样本占被检总数的87.77％;奶水牛血浆和乳清的TAC分别为2.44±1.04和3.78±1.68 U/mL,MDA含量分别为2.07±0.48和1.55±0.52 nmol/mL,其差异均达极显著水平（P＜0.01）.乳清TAC随乳汁SCC的升高而呈下降趋势,二者间呈显著负相关（r=-0.168,P＜0.05）;血浆MDA与乳清MDA间呈显著正相关（r=0.189,P＜0.05）,而血浆MDA与血浆TAC（r=0.391）、乳清MDA与血浆TAC（r=0.315）间呈极显著正相关（P＜0.01）.结论]乳清TAC可作为检测奶水牛乳房健康及其乳汁质量的备选指标.

Correlation between milk somatic cell count and antioxidant parameters in dairy buffalo cows

Objective]The correlationship between somatic cell count （SCC） and antioxidant parameters was analyzed so as to provide scientific references for milk quality monitoring and healthy breeding of dairy buffalo.Method]A total of 139 dairy buffalo cows were randomly chosen from one buffalo farm in Nanning.Milk and blood samples of each buffalo were collected simultaneously.Milk SCC was analyzed by FOSS instrument,and the serum and whey contents of total antioxidant capacity（TAC） and malondialdehyde （MDA） were detemined using Ferric Reducing Ability of Serum（FRAP） method and Thiobarbituric acid （TBA） method,respectively.Result]Out of the tested 139 buffaloes,12.23％ of buffalo cows had milk SCC ≥50.00×104/mL （as diagnostic criterion for subclinical mastitis）,the remainder 87.77％ of buffalo had SCC ＜ 50.00× 104/mL.Serum and milk content of TAC were 2.44±1.04 and 3.78±1.68 U/mL,respectively.Those of MDA were 2.07±0.48 and 1.55±0.52 nmol/mL,respectively.Both TAC and MDA had very significant differences between blood and milk （P＜0.01）.Milk serum content of TAC clearly decreased with milk SCC increase.A significant negative correlation existed between milk TAC content and milk SCC（r=-0.168,P＜0.05）.There was a significant positive correlation between blood MDA and milk MDA（r=0.189,P＜0.05）.An extraordinary significant positive correlation was found between blood TAC and blood MDA （r=0.391,P＜0.01）,and also between blood TAC and milk MDA （r=0.315,P＜0.01）.Conclusion]Milk TAC content can be used as an alternative parameter for monitoring buffalo udder health and milk quality in future.