番木瓜实生苗茎段组培快繁条件的优化

【目的】探讨不同激素对番木瓜实生苗茎段分化的影响,为提高番木瓜繁育效率提供参考依据。【方法】番木瓜种子采用直接接种、无菌水处理18 h后接种等4种方式预处理获得最佳种子处理方式;再以获得的实生苗茎段为外植体进行芽诱导培养基、增殖壮苗培养基、生根培养基的优化筛选。【结果】经6-BA 1.0 mg/L与GA31.0 g/L等体积混合液浸泡18 h后接种的番木瓜种子发芽率高达93.3%,接种培养后污染率低至3.3%;适合番木瓜茎段芽诱导培养基为MS＋6-BA 0.5 mg/L＋NAA 0.2 mg/L,增殖壮苗培养基为MS＋6-BA 0.2 mg/L＋NAA 0.1 mg/L＋蔗糖40.0 g/L,增殖系数最高达4.3,生根诱导培养基为MS＋IBA 1.0 mg/L＋KT 0.05 mg/L＋AC 2.0 g/L。【结论】MS＋6-BA 0.5 mg/L＋NAA 0.2 mg/L、MS＋6-BA 0.2 mg/L＋NAA 0.1 mg/L＋蔗糖40.0 g/L和MS＋IBA 1.0 mg/L＋KT 0.05 mg/L＋AC 2.0 g/L分别作为番木瓜实生苗茎段芽诱导、增殖壮苗和生根诱导培养基,可提高番木瓜实生苗茎段组培育苗效率。

Optimization of conditions for fast propagation of papaya seedling stem

【Objective】The effects of hormones on differentiation of papaya seedling stem were explored to provide technical references for large-scale fast propagation of papaya. 【Method 】The best seed treatment method was screened out of four different treatments,then the seedlings stems were used as explants to optimize the inducing medium,proliferation medium and rooting medium. 【 Result 】 Seeds soaked in equal volume mixture of 6- BA1. 0 mg / L and GA31.0 g/L for 18 h before sowing obtained the highest germination rate at 93.3 % and low rate of pollution at 3.3%. Induction medium MS ＋6-BA0.5 mg/L ＋NAA0.2 mg/L was suitable for papaya stem bud differentiation. The optimum medium for proliferation culture was MS ＋6-BA 0.2 mg/L ＋NAA 0.1 mg/L ＋sucrose 40.0 g/L,in which the proliferation coefficient reached 4.3, and the optimum medium for root inducing was MS＋IBA1.0 mg/L＋KT0.05 mg/L＋AC 2.0 g/L.【 Conclusion 】 MS ＋ 6- BA 0. 5 mg / L ＋ NAA 0. 2 mg / L, MS ＋ 6- BA 0. 2 mg / L ＋ NAA 0. 1 mg / L ＋ sucrose 40. 0 g / L and MS ＋IBA 1.0 mg/L ＋KT 0.05 mg/L ＋AC 2.0 g/L can be used as inducing medium,proliferation medium and rooting medium respectively for tissue culture and fast propagation of papaya seedling stem.