马尾松不同种源氨基酸含量与抗松材线虫病的关系

在选出和验证抗病马尾松种源的基础上，研究了马尾松不同抗病性种源的树体内氨基酸含量的差异。结果表明，抗病种源在接种松材线虫前后均比较感种源含有较少的组氨酸、苏氨酸、甘氨酸、异亮氨酸、胱氨酸、甲硫氨酸、亮氨酸、酪氨酸和精氨酸等９种游离氨基酸。它们与感病指数均呈正相关，这说明游离氨基酸含量低的马尾松种源抗松材线虫病。１１种固态氨基酸的含量与感病指数有负相关性，说明其含量较高的种源具较强抗病性能。

Studies on the Relationship of Amino Acid Content of Different Masson Pine Provenances and Their Resistance to Pine Wood Nematode (PWN)

On the basis of selected and tested resistance of masson pine (Pinus massoniana) provenances, and external symptoms of resistant difference of masson pine provenances, GX2(H), SC2(M), SX1(L) and black pine(S) (P.thunbergii) resistant to PWN (Bursaphelenchus xylophilus) were observed.The resistant provenance GX2 was stable (IR=100.0).However, those of the other provenances SC2, SX1 and black pine varied (IR=60.0, 40.0 and 0 respectively).Comparing with SC2, SX1 and black pine, the resistant factors in wood of GX2 were analysed and the results showed that:the factor that less sucking stimulatory of free amino acid (including His, Thre, Gly, Ile, Glu, Cys, Meth, Leu, Tyr, Try and Arg) existed in the wood of GX2 indicated it seemed to be one of the anti feeding causes.A clear positive correlation was found between the extrernal symptoms of index of pathogenesis (IP) and the content of free amino acid.It indicated that the pines with low content of free amino acid are resistant to PWN and vice versa.And a negative correlation between the IP and the total crude protein and 11 stationary amino acids were also found.