| 鸡肝脏和肌肉组织中常山酮残留的ELISA检测 |
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| 引用本文： | 吴宁鹏,杜向党,沈建忠.鸡肝脏和肌肉组织中常山酮残留的ELISA检测[J].畜牧兽医学报,2006,37(6):597-602. |
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| 作者姓名： | 吴宁鹏 杜向党 沈建忠 |
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| 作者单位： | 1. 中国农业大学动物医学院,北京,100094;河南省兽药监察所,郑州,450008
2. 中国农业大学动物医学院,北京,100094 |
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| 基金项目： | 国家自然科学基金项目（30130140）;致谢：感谢Intervet公司和美国农业部RossBeier博士提供了Hal标准品并给予技术指导. |
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| 摘 要： | 将常山酮进行人工改造,制备了半抗原常山酮琥珀酸衍生物（Hal-suc）.采用N-羟基琥珀酰亚胺活性酯法将半抗原与牛血清白蛋白（BSA）、卵清白蛋白（OVA）偶联,制备免疫原和包被原.动物免疫6次后采血制备抗血清,以间接ELISA法测定血清效价,测得抗血清的最佳工作浓度为1∶102 400.建立了常山酮在鸡肝脏和肌肉组织中检测的ELISA法,该方法在50、100、500 ng/g 的添加浓度水平下,在鸡肝脏和肌肉组织中测得的平均回收率范围分别为74.2%～96.8%和74.3%～90.0%,检测限分别为28和19 ng/g.
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| 关 键 词： | 常山酮 鸡肝脏 鸡肌肉 残留 酶联免疫吸附测定法 |
| 文章编号： | 0366-6964（2006）06-0597-06 |
| 收稿时间： | 2005-01-24 |
| 修稿时间： | 2005-01-24 |
Detection of Halofuginone in Chicken Liver and Chicken Muscle by Enzyme Linked ImmunoSorbent Assay |
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| WU Ning-peng,DU Xiang-dang,SHEN Jian-zhong.Detection of Halofuginone in Chicken Liver and Chicken Muscle by Enzyme Linked ImmunoSorbent Assay[J].Acta Veterinaria et Zootechnica Sinica,2006,37(6):597-602. |
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| Authors: | WU Ning-peng DU Xiang-dang SHEN Jian-zhong |
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| Institution: | 1. College of Veterinary Medicine, China Agricultural University, Beijing 100094,China; 2. Henan Provincial Supervising Agency of Veterinary Drugs, Zhengzhou 450008, China |
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| Abstract: | The halofuginone was modified to prepare Hal-suc as hapten.The hapten was conjugated to BSA as immunogen and to OVA as coated antigen by NHS ester procedure.The anti-Hal serum was acquired in rabbits against the conjugate after sixth immunization.The optional dilution of the anti-Hal serum was 1:102 400 by the indirect ELISA.An ELISA method for the determination of the Hal in chicken liver and muscle tissues was developed.In the chicken liver tissue fortified at 50,100,500 ng/g,the average recoveries ranged from 74.2% to 96.8%.In the chicken muscle tissue fortified at 50,100,500ng/g,the average recoveries ranged from 74.3% to 90.0%.The detection limits in chicken liver tissue and in chicken muscle tissue were 28ng/g and 19ng/g respectively. |
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| Keywords: | halofuginone chicken liver chicken muscle residues enzyme-linked immunosorbent assay(ELISA) |
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