Physical extent,frequency, and intensity of phosphatase activity varies on soil profiles across a Douglas-fir chronosequence |
| |
| Institution: | 1. Faculty of Forestry, University of British Columbia Vancouver, BC, Canada V6T 1Z4;2. Biology Department, University of British Columbia Okanagan, Kelowna, BC, Canada V1V 1V7;1. Iwade Research Institute of Mycology Co., Ltd., 1-9 Suehiro, Tsu, Mie 514-0012, Japan;2. Department of Immunology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan;3. Faculty of Agriculture, University of Miyazaki, 1-1 Nishi, Kibana-dai, Miyazaki 889-2192, Japan;1. INRA (Institut National de la Recherche Agronomique), Domaine de Gotheron, Unité Expérimentale Recherches Intégrées, 26320 St. Marcel-les-Valence, France;2. GRAB (Groupe de Recherche en Agriculture Biologique), Domaine de Gotheron, 26320 St. Marcel-les-Valence, France;3. INRA, Domaine Saint-Paul, Site Agroparc, Unité Plantes et Systèmes de Culture Horticoles, 84914, Avignon Cedex 9, France;1. Department of Geography, University Toulouse II, GEODE UMR 5602 CNRS, France;2. Institut d''Etudes Politiques (IEP), Toulouse, France;3. Institut de Recherche pour le Développement (IRD), Vientiane, Lao People''s Democratic Republic;4. National Agriculture and Forestry Research Institute (NAFRI), Vientiane, Lao People''s Democratic Republic;5. Géosciences Environnement Toulouse (GET), UMR 5563 CNRS, France;1. Pharmacy Department of Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China;2. National Engineering Laboratory for Anti-tumor Protein Therapeutics, School of Life Sciences, Tsinghua University, Beijing, 100084, China;3. National Center for Safety Evaluation of Drugs, National Institute for Food and Drug Control, Beijing, 100176, China;1. Hydrogéochimie et Interactions Sol-Environnement (HydrISE), UP.2012.10.102, Institut Polytechnique UniLaSalle, 19 rue Pierre Waguet, FR-60026 Beauvais, France;2. Laboratoire d’Ecologie végétale et Biogéochimie, Université Libre de Bruxelles, 50 Avenue F. Roosevelt, BE-1150 Brussels, Belgium;1. Sustainable Forest Management Research Institute UVa-INIA, Área de Ecología, E.T.S. de Ingenierías Agrarias de Palencia, Universidad de Valladolid, Campus La Yutera, Avda. de Madrid 44, 34071 Palencia, Spain;2. Área de Ecología, Facultad de Biología, Universidad de Salamanca, Campus Miguel de Unamuno, 37071 Salamanca, Spain |
| |
| Abstract: | In forest soils, the availability of phosphate is largely dependent on phosphatase activity. We used soil imprinting to compare in situ activity and fine-scale distribution of phosphatase on soil profiles located across forest chronosequences of four age classes young (5–6 yrs), canopy closure (24–30 yrs), stem exclusion (61–71 yrs), and older (90–103 yrs) of mixed Douglas-fir/paper birch stands regenerated after fire or clearcutting in southern interior British Columbia, Canada. Chromatography paper treated with a mixture of substrate and colorimetric reagent was applied directly to vertical soil surfaces, accessed through root windows. Stands older than 61 years had both the highest level of in situ phosphatase activity and larger, more intense regions of activity. Bray-extractable phosphorus was negatively related to imprintable phosphatase activity. We compared the changes in phosphatase activity with differences in the ectomycorrhizal fungal (EMF) community that had been documented previously in the same stands. Of 84 ectomycorrhizal fungi found on roots in at least two of the stand-age classes, eight taxa were positively correlated and one taxon (Rhizopogon vinicolor/vesiculosus) negatively correlated with high phosphatase activity. The frequency of three taxa appeared to be positively correlated with larger areas of activity on the soil profiles. By using an imprinting approach, this study was able to demonstrate, for the first time, that in situ phosphatase activity and physical attributes of that activity (i.e., number, size, and relative rates of each area of activity) were related to concentrations of soil nutrients and with the frequency of individual ectomycorrhizal fungi. |
| |
| Keywords: | Phosphatase Soil microsites Nutrient cycling Root windows Soil imprinting Ectomycorrhizal fungi Forest succession |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
