硒代蛋氨酸对肥育猪血浆和组织硒含量及抗氧化能力的影响

【目的】研究探讨硒代蛋氨酸对肥育猪血浆和组织硒含量及抗氧化能力的影响。【方法】选用64头体重约60kg的杜×长×大三元杂交肥育猪,随机分为4组,每组4个重复。饲粮硒添加水平分别为0(对照组)、0.15mg·kg-1(硒代蛋氨酸)、0.30mg·kg-1(硒代蛋氨酸)、0.30mg·kg-1(亚硒酸钠)。饲养至95kg左右结束试验。【结果】(1)添加硒组血浆、肝脏和背最长肌硒含量极显著高于对照组(P0.01),高水平硒代蛋氨酸硒组显著高于其它两个添加硒组(P0.05)。(2)饲粮添加硒代蛋氨酸提高了肥育猪血浆GSH-Px、CAT活性和T-AOC,降低了MDA含量(P0.05)。同时,硒代蛋氨酸组SOD活性和低水平硒代蛋氨酸组CAT活性显著高于亚硒酸钠组。硒代蛋氨酸或亚硒酸钠提高了肥育猪背最长肌宰后45minGSH-Px活性,降低羰基蛋白质含量(P0.05)。硒代蛋氨酸可降低宰后各时间点背最长肌MDA含量,宰后45min和72h也低于亚硒酸钠组(P0.05)。(3)硒处理组背最长肌GSH-Px基因相对表达量显著高于对照组(P0.05),低水平硒代蛋氨酸硒组SelW基因相对表达量显著高于对照组和高水平亚硒酸钠硒组(P0.05)。【结论】硒代蛋氨酸在血浆和组织中硒沉积率较高,抗氧化作用较强。

Effects of Selenomethionine on Selenium Concentration in Plasma and Tissues and Antioxidant Capacity of Finishing Pigs

Objeetive] The experiment was conducted to investigate the effect of diet supplemented with selenomethionine(Se-Met) on plasma and tissue selenium content and antioxidant capacity of finishing pigs.Method] Sixty-four crossbred (Duroc×Landrace×Largewhite)pigs(initial BW,60 kg) were randomly allocatedinto 4 treatments on the basis of weight following a randomized complete block design.Four replicates were designed for each treatment.Pigs were fed with corn-soybean meal-based diets supplemented with 0(control) or 0.15 and 0.30 mg·kg-1 Se from Se-Met or 0.30mg·kg-1 Se from sodium selenite (SS),respectively.One barrow per pen was slaughtered when all the pigs were weighed about 95kg on average.Result] All Se-added groups showed significantly increased Se concentration in plasma,liver and longissimus muscle compared with the control(P＜0.01),also 0.30 mg·kg-1 Se (Se-Met) group compared to 0.15mg·kg-1 Se (Se-Met)group or SS group (P＜0.05).Adding Se-Met significantly raised plasma glutathione peroxidase (GSH-Px) activity,catalase(CAT)activity,total antioxidant capaeity (T-AOC),and significantly decreased plasma malondialdehyde (MDA) content (P＜0.05).Diet supplemented with Se-Met significantly improved liver GSH-Px,CAT,SOD activity and T-AOC(P＜0.05).All Se-Met groups presented significantly higher liver SOD activity and 0.15 mg·kg-1 Se (Se-Met) significantly increased CAT activity with respect to SS ,Se-Met had greater absorption,deposition rate and antioxidant capacity in plasma and tissues.up(P＜0.05).Longissimus muscle GSH-Px activity at 45min post-slaughter was significantly raised and carbonyl protein content was significantly reduced in all Se-added groups(P＜0.05).Longissimus muscle MDA content at different times after slaughter was significantly decreased in all Se-Met groups compared to control.also 0.30mg·kg-1 Se(se-Met)group compared to SS group at 45min and 72h post-slaughter (P＜0.05).GSH-Px mRNA relative expression of longissimus muscle was significantly increased in all Se-added groups than that of the control (P＜0.05).0.15mg·kg-1 Se(Se-Met)group significantly increased SelW mRNA relative expression compared to both controland SS groups(P＜0.05).Conclusion] Compared with the same Se supplementation in the form of SS,Se-Met had greater absorption,deposition rate and antioxidant capacity in plasma and tissues.