中药方剂白头翁汤抗细菌内毒素的作用研究

探讨中药方剂白头翁汤对细菌内毒素（LPS）的治疗和预防作用,取中药饮片按常规方法煎制成生药质量浓度为1g/mL的白头翁汤药液。分高（1g/mL）、中（0.5g/mL）、低（0.1g/mL）3个剂量给小鼠灌胃,每天1次,连续5d。第1次给药前3h和最后1次给药后3h分别腹腔注射LPS（9mg/kg）。记录LPS攻毒后48h内小鼠的死亡率,同时测定各组小鼠的体重、血液指标及器官指数。结果显示,预防高剂量组的小鼠死亡率极显著低于LPS对照组,差异极显著,治疗高剂量组和预防中剂量组显著低于LPS对照组,差异显著。治疗高剂量组和预防高剂量组的小鼠体重在24h时显著高于LPS对照组,差异显著。治疗高剂量组的小鼠白细胞数目、血红蛋白浓度、平均血红蛋白和血小板升高数量较LPS对照组均差异显著。治疗高剂量组小鼠的肝脏、脾脏和肠管指数显著低于LPS对照组,均差异显著。表明中药方剂白头翁汤具有明显的抗大肠杆菌内毒素作用。

Study on the antiendotoxin action of Pulsatillae Decoction

The purpose of this research was to investigate the antiendotoxin action of Pulsatillae Decoction (PD).PD was extracted and condensed into the decoction at the crude drug concentration of 1 g/mL.Mice in prevention groups were challenged with 9 mg/kg lipopolysaccharide (LPS) by peritoneal injection after the treated with PD at three different concentrations (0.1,0.5 and 1 g/mL) for 5 days,and differently,mice in treatment groups were challenged with 9 mg/kg LPS by peritoneal injection before treated with PD at three concentrations (0.1, 0.5 and 1 g/mL) for 5 days.The determined items and results were as follow:compared with LPS control group,the mortality were significantly decreased in the middle- and high-dose prevention groups and high-dose treatment group;the body weight were significantly increased in the high-dose treatment and prevention groups at 24 h;the WBC,HGB,MCH and PLT were significantly increased in the high-dose treatment group;the index of liver,spleen and intestinal were significantly decreased in the high-dose treatment group.It suggested PD had significant antiendotoxin effect.