| 用溶血素从感染红细胞分离羊泰勒虫裂殖子 |
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| 引用本文： | 李有全,罗建勋,关贵全,马米玲,高金亮,刘志杰,党志胜,刘爱红,刘军龙,任巧云,鲁炳义,白启,殷宏. 用溶血素从感染红细胞分离羊泰勒虫裂殖子[J]. 畜牧兽医学报, 2007, 38(10): 1093-1097 |
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| 作者姓名： | 李有全 罗建勋 关贵全 马米玲 高金亮 刘志杰 党志胜 刘爱红 刘军龙 任巧云 鲁炳义 白启 殷宏 |
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| 作者单位： | 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室,兰州,730046 |
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| 基金项目： | 国家“863”计划(2006AA10A207)，国家自然科学面上项目(30571397)，欧盟EPIZONE、ICTTD和SSA-INCOME项目(INCONo.510561) |
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| 摘 要： | 用嗜水气单胞菌产生的细胞毒素—溶血素Ah-1,裂解羊泰勒虫感染的红细胞,通过Percoll密度梯度离心分离羊泰勒虫裂殖子,建立了一种自感染红细胞中分离泰勒虫裂殖子的快速方法。光学显微镜观察显示,所分离的羊泰勒虫裂殖子基本保留原有的正常形态,并从宿主细胞成分中游离。
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| 关 键 词： | 羊泰勒虫 裂殖子 溶血素 嗜水气单胞菌 |
| 文章编号： | 0366-6964（2007）10-1093-05 |
| 修稿时间： | 2006-12-21 |
Purification of Merozoites of Theileria spp. from Infected Erythrocytes in Sheep Using Hemolysin |
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| LI You-quan,LUO Jian-xun,GUAN Gui-quan,MA Mi-ling,GAO Jin-liang,LIU Zhi-jie,DANG Zhi-sheng,LIU Ai-hong,LIU Jun-long,REN Qiao-yun,LU Bing-yi,BAI Qi,YIN Hong. Purification of Merozoites of Theileria spp. from Infected Erythrocytes in Sheep Using Hemolysin[J]. Chinese Journal of Animal and Veterinary Sciences, 2007, 38(10): 1093-1097 |
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| Authors: | LI You-quan LUO Jian-xun GUAN Gui-quan MA Mi-ling GAO Jin-liang LIU Zhi-jie DANG Zhi-sheng LIU Ai-hong LIU Jun-long REN Qiao-yun LU Bing-yi BAI Qi YIN Hong |
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| Affiliation: | Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences ,Lanzhou 730046, China |
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| Abstract: | A simple and rapid method for purifying merozoites of Theileria spp.from infected erythrocytes in sheep or goats was developed.Infected erythrocytes were effectively lysed using hemolysin Ah-1 produced by Aeromonas hydrophila,and the lysate was subjected to centrifugation in a Percoll discontinuous density gradient at common speed.Light microscopical study showed that the merozoites purified retained their intact basic structure and were free of infected erythrocyte membrane and other host-cell components. |
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| Keywords: | Theileria spp. merozoite hemolysin Ah-1 Aeromonas hydrophila |
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