| Cry1Ac蛋白降解菌株FJSB3的分离鉴定及降解特性 |
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| 引用本文： | 赵辉欣,刘阳,邢福国.Cry1Ac蛋白降解菌株FJSB3的分离鉴定及降解特性[J].核农学报,2011,25(5):922-926. |
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| 作者姓名： | 赵辉欣 刘阳 邢福国 |
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| 作者单位： | 巾阳农业科学院农产品加丁研究所／农业部农产品加工综合性重点实验室,北京,100193 |
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| 基金项目： | 转基因生物新品种培育科技重大专项(2008ZX08012-005) |
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| 摘 要： | 从转Cry1Ac基因水稻种植田土壤中,分离纯化得到1株能高效降解Cry1Ac蛋白的细菌FJSB3。通过表型特征、16S rDNA扩增和电镜观察,初步鉴定FJSB3为寡养单胞菌（Stenotrophomonas sp.）。FJSB3发酵液能降解粗Bt蛋白。通过单因素试验确定FJSB3降解水稻秸秆中Cry1Ac蛋白的最适条...
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| 关 键 词： | Cry1Ac蛋白 寡养单胞菌 鉴定 Bt蛋白降解 |
| 收稿时间： | 2011-01-06 |
| 修稿时间： | 2011-03-14 |
ISOLATION AND IDENTIFICATION OF Cry1Ac PROTEIN-DEGRADING BACTERIA AND ITS DEGRADATION CHARACTERISTICS |
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| ZHAO Hui-xin LIU Yang XING Fu-guo.ISOLATION AND IDENTIFICATION OF Cry1Ac PROTEIN-DEGRADING BACTERIA AND ITS DEGRADATION CHARACTERISTICS[J].Acta Agriculturae Nucleatae Sinica,2011,25(5):922-926. |
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| Authors: | ZHAO Hui-xin LIU Yang XING Fu-guo |
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| Institution: | ZHAO Hui-xin LIU Yang XING Fu-guo(Key Laboratory of Agricultural Products Processing and Quality Control,Ministry of Agriculture/Institute of Agro-Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193) |
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| Abstract: | A degradation of Cry1Ac protein in rice straw, bacterium FJSB3 capable of highly degrading Cry1Ac protein was isolated from the agricultural soil where the transgenic Cry1Ac rice was grown. The FJSB3 was identified preliminarily as Stenotrophomonas sp. by phenotype observation, 16S rDNA analysis and electron microscope. SDS-PAGE showed that FJSB3 could degrade Bt protein. Based on the single factor experiments, the optimal conditions of degrading Cry1Ac protein in rice straw were: 30℃, initial concentration of bacterium 2.0×106 CFU/ml, pH 7.0, C/N 25/1.Under these conditions, the degradation rate of Cry1Ac protein reached 92.86%. |
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| Keywords: | Cry1Acprotein Stenotrophomonas sp identification Bt protein degradation |
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