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Antiproliferation effects of 188Re labeled monoclonal antibody on prostate cancer cells in vitro
Authors:CHEN Wei-zhen  ZHANG Yong  LU Han-ping  LIANG Chang-sheng  XIE Yao  LIU Chang-zheng
Affiliation:1.Department of Nuclear Medicine,The Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,China;2.Department of Experimental Nuclear Medicine,The School of Preclinical Medicine,3The Basic Medical Experimental Teaching Center,4Department of Anatomy,The School of Preclinical Medicine,Sun Yat-sen University,Guangzhou 510080,China.E-mail:zy5040@163.com
Abstract:
AIM: To investigate the effect of 188Re labeled monoclonal antibody on prostatic specific membrane antigen 7E11C5.3,radioimmunotherapy for the treatment of human prostate cancer cell line LNCaP in vitro.METHODS: 188Re-7E11C5.3 was prepared by direct 2-mercaptoethanol reduction method.Labeling efficiency and radiochemical purity was measured by paper chromatography.Immunoreactive fraction was determined by linear extrapolation.Cytotoxicity to LNCaP cells was determined by MTT assay.RESULTS: The labeling yield of 188Re-7E11C5.3 was (93.16±2.18)%,the radiochemical purity was (95.62±0.48)%,and the immunoreactive fraction was (74.86±1.86)%.The inhibitory effect of 188Re-7E11C5.3 on cell proliferation of LNCaP cells was significantly higher than that of 188Re-mIgG or 188ReO-4.The 50% inhibitory doses (IC50) of 188Re-7E11C5.3,188Re-mIgG,and 188ReO-4 were (23.38±3.73)×107 Bq/L,(59.21±8.02)×107 Bq/L and (68.89±10.91)×107 Bq/L,respectively.CONCLUSION: 188Re-7E11C5.3 can effectively inhibit the growth of in vitro cultured prostate cancer cells and shows much potential for prostate cancer radioimmunotherapy.
Keywords:Prostatic neoplasms  Radioimmunotherapy  Antibodies  monoclonal  Prostate-specific membrane antigen  Rhenium-188  
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