Salviae miltiorrhizae injection antagonists renal-toxicity of streptomycin

AIM: To investigate the antagonistic and protective mechanism of salviae miltiorrhizae injection(SMI) on kidney toxicity damaged by streptomycin (SM). METHODS: 40 guined pigs were divided randomly into 4 groups: the control group, SM group, SMI+SM group and SMI group. Light microscope, transmission electron microscope (TEM), immunohistochemical staining, TdT mediated biotin dUTP nick-end cabelling (TUNEL) techniques and image quantitative analysis technique were used to observe the change of kidney after administration of SM and SMI. The expressions of iNOS and bFGF, apoptosis, and the activity of N-acetyl-D-glucosaminidase(NAG) were also observed. RESULTS: 10 d after administration of SM, the serious pathological change in SM group was observed under the light microscope and transmission electron microscope. Slight damage was found in SMI+SM group, and the number of apoptosis cells was fewer than that in SM group. The result of immunohistochemical implied that the iNOS expression in SM group was higher than that in SMI+SM group, while the bFGF expression was lower. The activity of NAG in SMI+SM group was lower than that in SM group (P<0.01). CONCLUSION: Salvia miltiorrhiza injection inhibits the increased expression of iNOS during kidney toxicity damaged by SM, reduces the activity of NAG, increases the expression of bFGF, and decreases the apoptosis, hence has the antagonism to the kidney toxicity damaged by SM.