Effect of G-CSF on dendritic cell subsets in donors mobilized peripheral blood cells |
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Authors: | SHI Dun-yun WANG Ming-chun ZHANG Qiong-li LI Ming LIU Huang-xun ZHUO Jia-cai XU Yun |
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Institution: | 1.Institute of Hematology, 2 Department of Hematology, Shenzhen Second Peoples Hospital, Shenzhen 518039, China. E-mail: shidunyun@yahoo.com.cn |
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Abstract: | AIM: To explore the effect of granulocyte colony stimulating factor (G-CSF) on donor’s dendritic cell (DC)subsets in peripheral blood cells (PBC). METHODS: The subsets of dendritic cells in PBC were analyzed by CD34-Lin- HLA-DR+ cells and the levels of IL-12p40, IL-10, IFN-γ, IL-4 in the serum were tested by ELISA before (25 cases) or after G-CSF mobiling. The relationship between the ratio of DC1/DC2 (CD11c+CD123-/CD11c-CD123+) and CD34+/MNC was explored. RESULTS: CD34+/MNC in PBC harvests was above 0.4% in 23 cases, and the ratio of DC1/DC2 was lower, the HLA-DR expression of DC2 was enhanced after G-CSF mobiling than before, but the levels of IL-12p40, IL-10, IFN-γ, IL-4 in donors serum and CD83 expression of DC2 were not changed by G-CSF. CONCLUSION: DC2 increased accompanied by the increase in CD34+ cells in the PBC harvests. Although the expression of HLA-DR in DC2 was up-regulated by G-CSF, these DC2 did not regulate Th2 cells to excrete inhibitor cell factors and kept on the precursor characters. |
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Keywords: | Granulocyte colony stimulating factor Dendritic cells Stem cells transplantation Graft vs host disease |
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