| 中华卵索线虫tra-1基因cDNA片段的克隆及实时定量表达分析 |
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| 引用本文： | 贺俊飞,王伟娜,周青春,刘绪生,王国秀.中华卵索线虫tra-1基因cDNA片段的克隆及实时定量表达分析[J].植物保护学报,2009,36(4):324-328. |
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| 作者姓名： | 贺俊飞 王伟娜 周青春 刘绪生 王国秀 |
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| 作者单位： | 华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,武汉,430079 |
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| 基金项目： | 国家自然科学基金(30870350,30740064) |
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| 摘 要： | 差异(P>0.05).线虫在宿主体内不同的感染强度(40/1与10/1)对tra-1基因转录活性无显著影响(P>0.05).表明tra-1基因参与调控中华卵索线虫雌性生殖系统的发育、卵的形成以及胚胎的发育.
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| 关 键 词： | 昆虫病原线虫 索科 tra-1基因 3'-RACE 实时荧光定量PCR |
| 收稿时间： | 2008/10/6 0:00:00 |
cDNA cloning and quantitative analysis by real-time RT-PCR of tra-1 gene in Ovomermis sinensis |
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| He Junfei,Wang Wein,Zhou Qingchun,Liu Xusheng and Wang Guoxiu.cDNA cloning and quantitative analysis by real-time RT-PCR of tra-1 gene in Ovomermis sinensis[J].Acta Phytophylacica Sinica,2009,36(4):324-328. |
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| Authors: | He Junfei Wang Wein Zhou Qingchun Liu Xusheng and Wang Guoxiu |
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| Institution: | Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, Hubei Province, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, Hubei Province, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, Hubei Province, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, Hubei Province, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, Hubei Province, China |
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| Abstract: | |
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| Keywords: | entomopathogenic nematodes Mermithidae tra-1 gene 3'-RACE real-time quantitative RT-PCR |
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