| 棉酚对猪原代培养黄体细胞增殖和凋亡的影响 |
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| 引用本文： | 龙安梅,邬静,袁莉芸,袁慧. 棉酚对猪原代培养黄体细胞增殖和凋亡的影响[J]. 中国兽医学报, 2009, 29(10) |
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| 作者姓名： | 龙安梅 邬静 袁莉芸 袁慧 |
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| 作者单位： | 湖南农业大学,动物医学院,湖南,长沙,410128 |
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| 摘 要： | 采用长大杂母猪原代培养的黄体细胞,通过MTT法、DNA Ladder和TUNEL检测法,探讨了0、0.4、2、10、50 mg/L棉酚对猪原代培养黄体细胞增殖和凋亡的影响.结果表明,棉酚分别染毒24、48 h后,与对照组比较,各浓度组对黄体细胞增殖具有明显的抑制作用(P<0.05,P<0.01),黄体细胞的凋亡率达5%～23%;这种细胞毒性作用呈剂量相关性,在一定剂量下,具有时间依赖关系.
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| 关 键 词： | 醋酸棉酚 猪 原代培养 黄体细胞 细胞增殖 凋亡 |
Effect of gossypol on proliferation and apoptosis of primary luteal cells in sow |
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| LONG An-mei,WU Jing,YUAN Li-yun,YUAN Hui. Effect of gossypol on proliferation and apoptosis of primary luteal cells in sow[J]. Chinese Journal of Veterinary Science, 2009, 29(10) |
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| Authors: | LONG An-mei WU Jing YUAN Li-yun YUAN Hui |
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| Abstract: | This study was designed to determine the effect of gossypol at 0.4,2,10,50 mg/L on proliferation and apoptosis in primary luteal cells of Landrace-Yorkshire Hybrid sows by MTT and TUNEL assay.The results showed that 24 h and 48 h after treatment with gossypol,obvious inhibition effect on luteal cell proliferation was observed in each concentration group(P<0.05,P<0.01).The apoptosis percentage of luteal cells induced by gossypol was among 5% to 23% in each contamination group.Gossypol at high and low dose could inhibit the proliferation of luteal cells and induce their apoptosis.This role was dose-related,and in a certain dosage,a time-dependent manner. |
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| Keywords: | gossypol sow primary culture luteal cells cell proliferation apoptosis |
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