| 罗汉果种子传播小西葫芦黄化花叶病毒的检测 |
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| 引用本文： | 朱英芝,陈保善,蒙姣荣.罗汉果种子传播小西葫芦黄化花叶病毒的检测[J].广西农业生物科学,2011,30(6):678-681. |
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| 作者姓名： | 朱英芝 陈保善 蒙姣荣 |
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| 作者单位： | 1. 广西大学农学院,南宁,530004
2. 亚热带农业生物资源保护与利用国家重点实验室,南宁,530004 |
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| 基金项目： | 广西自然科学基金项目，广西创新能力建设项目(桂科能05112001-1B;桂科能05112001-1、共同资助感谢广西大学农学院植物病理研究室林纬老师提供罗汉果植株及种子材料.感谢评审人对本文提出的宝贵意见及做出的修改 |
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| 摘 要： | 为了解小西葫芦黄化花叶病毒（Zucchini yellow mosaicvirus，ZYMV）是否能通过罗汉果种子传播。本研究通过取ZYMV阳性的罹病罗汉果植株种子700粒，400粒直接播种于防虫网室中，300粒经表面消毒处理后播种于MS培养基上。最终获得213棵罗汉果实生苗。对所获得实生苗进行生物学鉴定及ZYMV特异性RT-PCR检测，均未检测到ZYMV阳性植株。结果表明ZYMV不能经罗汉果种子传播，或其传播率很低。
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| 关 键 词： | 罗汉果 小西葫芦黄化花叶病毒 RT-PCR 种子 |
Detection of Zucchini yellow mosaic virus Transmission through Seeds of Siraitia grosve norii |
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| Zhu Yingzhi,Chen Baoshan,Meng Jiaorong.Detection of Zucchini yellow mosaic virus Transmission through Seeds of Siraitia grosve norii[J].Journal of Guangxi Agricultural and Biological Science,2011,30(6):678-681. |
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| Authors: | Zhu Yingzhi Chen Baoshan Meng Jiaorong |
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| Institution: | 2,3, 1 College of Agriculture, Guangxi University, Nanning, 530004; 2 State Key Laboratory for Conservation and Utilization of Subtropical Agro-biore sources, Nanning, 530004; 3 College of Life Science and Technology, Guangxi University, Nanning, 530004 |
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| Abstract: | In order to understand whether the Zucchini yellow mosaic virus （ZYMV） transmitted through seeds of Siraitia grosvenorii. A total of 700 seeds were collected from ZYMV-infected plants. Four hundred of 700 seeds were cultivated directly in net-house for control pest, and 300 were cultivated in MS medium after surface steril- ization with alcohol. ZYMV of young plantlets were detected by observation or RT-PCR with ZYMV-specific primers. Two hundred and thirteen young plantlets were survived from 700 seeds. No ZYMV-positive sample was detected by observed or RT-PCR with ZYMV-specific primers. The results indicated that ZYMV could not be transmitted via seeds ofSiraitia grosvenorii, or the transmission rate was very low |
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| Keywords: | Siraitia grosvenorii Zucchini yellow mosaic virus RT-PCR Seeds |
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