| 红锥ISSR—PCR扩增体系的优化 |
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| 引用本文： | 刘海龙,董民利,杨开太,蒋华,覃子海.红锥ISSR—PCR扩增体系的优化[J].广西林业科学,2011,40(4):288-291. |
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| 作者姓名： | 刘海龙 董民利 杨开太 蒋华 覃子海 |
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| 作者单位： | 1. 广西林业科学研究院国家林业局中南速生材繁育实验室广西优良用材林资源培育重点实验室,南宁,530002
2. 广西国有维都林场,来宾,546100 |
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| 基金项目： | 广西林科院基本科研业务费项目 |
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| 摘 要： | 对广西红锥种群的DNA进行提取和ISSR—PCR扩增体系进行优化。分析了退火温度、模板DNA浓度、Mg^2＋浓度、dNTPs浓度、TaqDNA聚合酶用量对反应结果的影响。红锥ISSR—PCR分析较适宜的扩增体系是：25μL PCR反应体积中，buffer（10mM Tris-HCl，pH9．0，50mM KCl，0．1...
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| 关 键 词： | 红锥 ISSR 优化 遗传多样性 |
The Optimization of ISSR-PCR Reaction System for Castanopsis hystrix |
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| LIU Hai-long,DONG Min-li,YANG Kai-tai,JIANG Hua,QIN Zi-hai.The Optimization of ISSR-PCR Reaction System for Castanopsis hystrix[J].Guangxi Forestry Science,2011,40(4):288-291. |
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| Authors: | LIU Hai-long DONG Min-li YANG Kai-tai JIANG Hua QIN Zi-hai |
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| Institution: | 1(1.Guangxi Forestry Research Institute,Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China,Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation,Nanning 530002,China;2.Guangxi Weidu State Forest Farm,Laibin,Guangxi 546100,China) |
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| Abstract: | The DNA and ISSR-PCR amplification of Guangxi Catamopsis hystrix was extracted and optimized respectively. The factors which affected the ISSR amplification such as annealing temperature, template DNA dosage, Mg^2＋ concentration, dNTPs concentration and unit of Taq DNA polymerase were selected and optimized. The results showed that the reaction system being suitable for ISSR - PCR of Castanopsis hystrix was as follows ： 1 × Taq buffer （10 raM/L Tris-HCl , pH 9.0, 50 mM/L KC1 and 0.1% Triton X-100） , 1.25 U Taq DNA polymerase , 0.2 mM 4 8 dNTP, 0.4μM primers, 2.0 mM/L MgCl2 and 50 ng template DNA in total 25 μL reaction volume. |
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| Keywords: | Castanopsis hystrix ISSR optimization genetic diversity |
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