转基因棉的不同组织部位对甜菜夜蛾抗性的研究(摘要)(英文)

目的]转基因抗虫棉有效的控制了靶标害虫棉铃虫的为害,但是近年来甜菜夜蛾在棉田发生日益严重。方法]利用转基因棉33B、GK12和SGK321的花、倒1叶、倒3叶、倒6叶、倒14叶饲喂甜菜夜蛾初孵幼虫,分别在接虫后第3天、第7天、第10天、第16天和第19天记录幼虫存活、死亡情况以及其后的化蛹情况,化蛹后24h称蛹重。结果]甜菜夜蛾幼虫在转基因棉上不同部位的生存率曲线分布、化蛹率和蛹重与在常规棉对照的相应部位基本没有差异;同一棉花品种不同部位的叶片上的化蛹率没有差异,花上的化蛹率显著低于叶片;同一棉花品种叶片和花上的蛹重没有差异。结论]转基因棉对甜菜夜蛾的抗性不强。因此,在转基因棉田要特别关注甜菜夜蛾种群的发生态势和防治。

Insect Resistance of Different Tissues of Transgenic Cotton to Spodoptera exigua (Hübner)

Objective] The aim was to learn the resistance of different tissues and organs of transgenic cotton to Spodoptera exigua （Hbner）. Method] Flowers,the 1st,the 3rd,the 6th and the 14th leaves from the top of 33B,GK12 and SGK321 were used to feed S. exigua neonates respectively. Survival larvae and dead ones were counted on the 3rd,the 7th,the 10th,the 16th and the 19th day; meanwhile,the pupae amount was recorded,and the pupae weight was measured at the 24th h after pupation. Result] The survival curves,pupation rates and pupae weights of S. exigua feeding on different tissues of transgenic cotton were not significantly different from those of S. exigua feeding on the corresponding tissues of conventional cotton; pupation rate of S. exigua feeding on different leaves of the same cotton variety were not significantly different from each other,but all higher than that of S. exigua feeding on the flowers of that cotton; and there were no differences among pupation weights of S. exigua feeding on different leaves or flowers of the same cotton variety. Conclusion] Transgenic cotton showed weak resistance to S. exigua. Hence,in the transgenic cotton fields,more attention should be paid to occurrence trend of S. exigua and its control.