杀鲑气单胞菌杀日本鲑亚种胞外产物毒性及免疫原性分析

用玻璃纸平板法提取了杀鲑气单胞菌杀日本鲑亚种Aeromonassal monicida masoucida的胞外产物（ECP）。毒性试验证实，ECP对剑尾鱼Xiphophorus helleri具有致死性，其半致死量（LD50）为4.72μg蛋白/g体重。SDS-PAGE表明，ECP由13条蛋白带组成。利用大鼠抗ECP血清进行的Western-blot印迹显示，组成ECP的13条蛋白带中有7条具有免疫原性，能够引发机体的免疫应答反应产生抗体，其分子量分别为88、70、42、39、36、22和15kDa。

Analysis of virulence and immunogenicity of the extracellular products (ECP) extract from Aeromonas salmonicida masoucida

The extracellulose products （ECP） of Aeromonas salmonicida masoucida was extracted by using cellophane plate technique. It was proved to be lethal to swordtail fish （Xi phophorus helleri）, and the LD50 does was 4.72 g protein/g. The ECP was analyzed by SDS- PAGE and thirteen proteins were observed. Western-blot with rat＇s antiserum against ECP showed that seven proteins among those thirteen proteins, whose molecular weight were 88, 70, 42, 39, 36, 22 and 15 kDa,respectively, were considered to have immunogenicity and could activate the immunoreaction of the hosts to secrete antibody.