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| 16S rRNA定量分析技术对绵羊瘤胃固相纤维降解菌的研究 | |
| 引用本文： | 薛丰,刘大程,王洪荣,卢德勋,高民.16S rRNA定量分析技术对绵羊瘤胃固相纤维降解菌的研究[J].中国饲料,2007(1):38-40. |
| 作者姓名： | 薛丰 刘大程 王洪荣 卢德勋 高民 |
| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,呼和浩特市,010018 2. 扬州大学动物科技学院 3. 内蒙古农牧科学院动物营养所 |
| 摘 要： | 本试验选择3只安装永久性瘤胃瘘管、体重25￣30kg的羯羊,使用特异性16SrRNA目标探针进行斑点杂交研究绵羊瘤胃固相纤维降解菌的动态变化规律。结果表明:3种纤维分解菌的RNA占总菌数RNA的15.76%;在各时间点产琥珀酸拟杆菌的相对含量均高于黄化瘤胃球菌和白色瘤胃球菌相对含量。 |
| 关 键 词： | 纤维分解茵 斑点杂交 |
| 文章编号： | 1004-3314(2007)01-0038-04 |
Study on sheep rumen solid cellulolytic bacteria by 16S rRNA quantitative analytical techniques |
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| Xue Feng Liu Dacheng et al..Study on sheep rumen solid cellulolytic bacteria by 16S rRNA quantitative analytical techniques[J].China Feed,2007(1):38-40. | |
| Authors: | Xue Feng Liu Dacheng |
| Institution: | Xue Feng Liu Dacheng et al. |
| Abstract: | Three 25 ~ 30 kg sheep with ruminal cannula were used to study variety disciplinarian of solid attached ruminal predominant cellulolytic bacteria.Three cellulolytic bacteria was quantified by dot-blot hybridisation using specific 16S rRNA-targeting probes.The sum of RNA of the three main cellulolytic bacterial species represented on average 15.76 % of the total bacterial RNA.In all samples,the relative population size of F.succinogenes was higher than that of R.albus and R.flavefaciens. |
| Keywords: | 16S rRNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |