AM真菌对红花生长及其有效成分的影响

丛枝菌根(Arbuscular mycorrhizas,AM)真菌是土壤生态系统中广泛分布的优秀微生物资源,是具有重要经济价值的药用植物。本文基于人工培育条件下对中药材红花(Carthamus tinctorius)分别进行摩西球囊霉(Glomus mosseae)、根内球囊霉(Glomus intraradices)和混合菌(G. mosseae,Glomus etunicatum,Glomus microagregatum,Glomus caledonium,Glomus cladoideum和G. intraradices) 3种AM真菌接种处理,并以接种等量灭活菌种作为对照组处理。采用高效液相色谱法测定不同采摘时期红花绒中羟基红花黄色素A和山奈素含量的变化。结果表明:与对照组相比,AM真菌接种处理显著促进了红花生物量的积累,且AM真菌混合接种效果显著高于AM真菌单接种;在次生代谢产物合成方面,AM真菌接种处理以及不同采摘期的红花中羟基红花黄色素A和山奈素的含量差异不显著。表明AM真菌虽能促进红花的生长,但不影响羟基红花黄色素A和山奈素的合成。

Effects of Arbuscular Mycorrhizal Fungi on Growth and Active Constituents of Carthamus tinctorius

Arbuscular mycorrhizas (AM) fungi is widely distributed excellent microbial resources in soil ecosystems and it is often used in medicinal plants with important economic value. In this study,Carthamus tinctorius was inoculated with different AM fungi: Glomus mosseae,Glomus intraradices and Mixed AM fungi (G. mosseae,G. etunicatum,G. microagregatum,G. caledonium,G. cladoideum and G. intraradices),respectively and inoculated with the same amount of inactivated bacteria as the control group. The inoculation effect of AM fungi was identified by measuring the infection rate and spore density,and the content of HSYA and kaempferol in C. tinctorius velvet was determined by high performance liquid chromatography at different picking stages. The purposes of this study were to investigate the effects of AM fungi on the growth and active constituents of C. tinctorius,and to analyze the effects of different flowering stages on HSYA and kaempferol so as to provide an important theoretical basis for promoting agricultural production of C. tinctorius in Xinjiang. We measured the content of hydroxysafflor yellow A and Kaempferol in C. tinctorius velvet by the high performance liquid chromatography method at different picking stages. The results showed that AM fungi could significantly promote the accumulation of aboveground parts,underground roots and floral biomass of C. tinctorius. In terms of the synthesis of secondary metabolites,there was no significant difference in the content of hydroxysafflor yellow A and Kaempferol in C. tinctorius under different AM fungi treatments and different growth stages.