AFLP analysis of genetic diversity within and between populations of perennial ryegrass (Lolium perenne L.) |
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| Authors: | KM Guthridge MP Dupal R K?lliker ES Jones KF Smith and JW Forster |
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| Institution: | (1) Plant Biotechnology Centre, Agriculture Victoria, La Trobe University, Bundoora, Victoria, 3089, Australia;(2) CRC for Molecular Plant Breeding, Australia;(3) Pastoral and Veterinary Institute, Agriculture Victoria Hamilton, Private Bag 105, Hamilton, Victoria, 3300, Australia |
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| Abstract: | Amplified fragment length polymorphism (AFLP) analysis has been used to measure genetic diversity in perennial ryegrass (Lolium perenne L.) and to relate intra- and interpopulation variation to breeding history. Cluster analysis of AFLP data from contrasting
populations showed features consistent with the origins of these varieties. Significant differences in intrapopulation diversity
were detected and partial separation of different cultivars was observed. Restricted base cultivars, derived from small numbers
of foundation clones, were suitable for this type of study, allowing near complete discrimination of closely related cultivars.
Analysis of bulked samples was based on the pooling of genomic DNA from 20 individuals from 6 selected populations. Cluster
analysis of AFLP data from bulked samples produced a phenogram showing relationships consistent with the results of individual
analysis. AFLP profiling provides an important tool for the detection and quantification of genetic variation in perennial
ryegrass.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | AFLP phenogram genetic diversity molecular marker Lolium perenne L |
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