小麦种质抗白粉病基因的分子标记检测 |
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引用本文: | 王玉叶,潘艳秋,靳婷.小麦种质抗白粉病基因的分子标记检测[J].安徽农业科学,2013,41(10):4276-4279. |
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作者姓名: | 王玉叶 潘艳秋 靳婷 |
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作者单位: | 农业部黄淮南部小麦生物学与遗传育种区域重点实验室,安徽省作物生物学重点实验室,安徽农业大学农学院,安徽合肥230036;安徽省淮南农场农科所,安徽淮南232035;农业部黄淮南部小麦生物学与遗传育种区域重点实验室,安徽省作物生物学重点实验室,安徽农业大学农学院,安徽合肥230036 |
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基金项目: | 现代农业产业技术体系,国家科技支撑计划 |
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摘 要: | 目的]促进小麦抗白粉病基因材料在小麦育种及生产中的应用。方法]利用STS、SCAR和SSR标记有效检测124个小麦品种资源中Pm21、Pm30、Pm34 3个类型抗白粉病基因的分布。结果]124份亲本材料中,7份材料含有Pm21基因(占5.65%),16份材料含有Pm30基因(占12.9%),4份材料含有Pm34基因(占3.23%);以2种抗性基因聚合形式存在的共有2种类型:其中1份材料含有Pm21+Pm30(占0.81%),1份材料含有Pm30+Pm34(占0.81%);没有发现以Pm21+Pm34 2种抗性基因聚合形式和Pm21+Pm30+Pm34 3种抗性基因聚合形式存在的类型。结论]利用与抗白粉病基因Pm21、Pm30和Pm34连锁的特异标记对品种资源进行检测,可为小麦育种提供广谱、持久抗病的新材料。
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关 键 词: | 小麦 白粉病 分子标记 辅助选择 |
Detection of Powdery Mildew Resistance Genes in Wheat Germplasm Using Molecular Markers |
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Institution: | WANG Yu-ye et al(Key Laboratory of Wheat Biology and Genetic Breeding of Ministry of Agriculture in the South of Huanghuai Area,Anhui Province Key Laboratory of Crop Biology,Agronomy College of Anhui Agricultural University,Hefei,Anhui 230036) |
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Abstract: | Objective] In order to promote the use of the wheat powdery mildew resistance genes in wheat breeding and production.Method] The distribution of Pm21,Pm30,and Pm34 genes in 124 wheat variety resources using STS,SCAR and SSR molecular markers was detected.Result] The results showed that the frequency of Pm30 gene(16 wheat varieties,12.9%) was highest,Pm21 was higher(7 wheat varieties,5.65%) and Pm34 was lowest(4 wheat varieties,3.23%) in 124 wheat variety resources.What's more,the research showed that part of wheat variety resources contained two kinds of wheat powdery mildew resistance genes combination,and their distribution frequency was also different.One material(0.81%) was detected to contain Pm21 and Pm30.One(0.81%) carried Pm30 and Pm34.We did not find Pm21 + Pm34 two resistance genes aggregation form or Pm21+ Pm30 + Pm34 three types of resistance genes polymerization form.Conclusion] We detected variety resources with powdery mildew resistance gene of Pm21 or Pm30 or Pm34 chaining specific PCR markers,so as to provide broad spectrum and durable resistance materials for wheat breeding. |
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Keywords: | Wheat Powdery mildew Molecular marker Assisted selection |
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