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以大蜡螟与人工培养基连续传代的两种斯氏线虫生物学特性比较
引用本文:徐洁莲,谢汝创.以大蜡螟与人工培养基连续传代的两种斯氏线虫生物学特性比较[J].昆虫天敌,1992(2).
作者姓名:徐洁莲  谢汝创
作者单位:广东省昆虫研究所,广东省昆虫研究所
摘    要:本文比较了 Steinernema carpocaprae 和 Steinernema anomali 两个种(品系)的斯氏昆虫病原线虫,分别在大蜡螟和鸡什海绵人工培养基下连续传代培养的两个种群的形态、带菌率、发育、繁殖、毒力和寻找寄主能力的差异。试验表明:同一种(品系)长期在蜡螟传代的线虫种群比人工培养基传代的种群的线虫体较大,在寄主昆虫内发育怏、繁殖量大。但细菌保留率、感染率、感染指数、在沙里寻找寄主并使其致死的能力稍低。上述的差异在 S.anomali 这个种尤为明显,S.car pocapsae 则差异不大。

关 键 词:昆虫病原线虫  连续传代培养  大蜡螟  鸡什人工培养

BIOLOGICAL COMPARISONS FOR TWO SPECIES OF ENTOMOPATHOGENIC NEMATODES IN WAX MOTH AND ARTIFICIAL MEDIUM
Xu Jielian Xie Ruchuang.BIOLOGICAL COMPARISONS FOR TWO SPECIES OF ENTOMOPATHOGENIC NEMATODES IN WAX MOTH AND ARTIFICIAL MEDIUM[J].Natural Enemies of Insects,1992(2).
Authors:Xu Jielian Xie Ruchuang
Institution:Guangdong Entomological Institute
Abstract:The comparisons of morphology retaing rate of symbiotic,development,parog- ation,toxity and the ability for seeking host have been made rearing successive- ly in various food for two species of entompathogenic nematodes:Steinernema carpocapsae and S.anomali.The food include:larvae of wax moth,chicken offal sponge with artifical medium.The results revealed that comparing with the artifical medium,The larvae of wax moth as food for nematodes had many ristic such as larger body size,higher developmental rate,more progenies. But the nematode cultured using the larvae of wax moth,retaing rate of symbiotic bacteria,incidence,infectivity index were lower than that reared in artifical medium.within two population of S.anomali the difference was obvious.
Keywords:Entomopathogenic nematodes  succession cultivation  wax moth  chicken offal medium
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