泡桐组培苗丛枝病原体PCR检测*

Detection ofMycoplasmalike Organism Associated With Paulownia TissueCultureWitches'''' -Broom by Polymerase Chain Reaction(PCR)

Thepolymerase chain reaction (PCR)was successfully employed to develop aspecific assay for plant pathogenic mycoplasmalike organism associated with paulowniawitches'-broom(PWB).A1. 2 kb DNA fragment was amplified from DNA extracted fromPWB diseased paulownia tissue culture by PCR. No amplified DNA was detected fromhealthy paulownia sample. Aminimum of only 2. 5 pg total nucleic acids from PWB MLO- in-fected paulownia tissue culture were needed to detect PWB MLO when a 1. 2 kb DNA frag-ment was amplified by PCR.Results showed that DNA amplification by PCR was a highsensitive method in detecting PWB MLO.