耐寡营养高效解磷菌株XMT-5的分离鉴定及解磷特性

为获得更加适应寡营养的自然环境条件的解磷菌株,以1/4蒙金娜培养基从土壤中分离筛选高效解磷菌株,并对其进行鉴定,分析其生长条件及解磷机制。结果表明,从土壤中共分离获得解磷菌9株,通过对解磷量的定量测定确定高效解磷菌株XMT-5,其最大解磷量为195.63 mg/L;从菌落特征、生理生化特性及16S rRNA基因序列分析三方面鉴定菌株XMT-5为根瘤菌(Rhizobium sp.);菌株XMT-5在寡营养条件下可以分泌柠檬酸、酒石酸和乙酸3种有机酸,从而可使培养液pH值下降;菌株XMT-5可在温度4~42℃、pH值4~10、Na Cl质量浓度0~80 g/L以及寡营养条件下生长,环境适应能力较强,具有良好的应用前景。

Isolation,Identification and Phosphorolysis Characteristics of High-efficient Phosphate-solubilizing Bacterium XMT-5 under Oligotrophic Conditions

In order to obtain strains which could adapt more oligotrophic natural environmental conditions,Monkina medium diluted by 4 times was used to isolate high-efficient phosphate-solubilizing bacteria,and the bacteria were identified,the growth condition and phosphorolysis mechanism were studied.The results showed that nine phosphate-solubilizing strains were screened out,and the high-efficient phosphate-solubilizing strain XMT-5 was determined through the quantitative determination of phosphate-solubilizing quantity,which could solubilizing phosphate 195.63 mg/L.According to the colony morphology,physiological and biochemical characteristics and 16S rRNA gene sequence analysis,strain XMT-5 was identified as Rhizobium sp.Under the oligotrophic condition,strain XMT-5 could secrete citric acid,tartaric acid and acetic acid,so lead to a drop of pH value.Strain XMT-5 could grow under the oligotrophic condition with 4-42 ℃,pH value of 4-10 and NaCl of 0-80 g/L.The strain had strong adaptability to the environment,and good application prospect.